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Detection of pro angiogenic and inflammatory biomarkers in patients with CKD
Journal article   Open access   Peer reviewed

Detection of pro angiogenic and inflammatory biomarkers in patients with CKD

Diana Jalal, Bridget Sanford, Brandon Renner, Patrick Ten Eyck, Jennifer Laskowski, James Cooper, Mingyao Sun, Yousef Zakharia, Douglas Spitz, Ayotunde Dokun, …
Scientific reports, Vol.11(1), pp.8786-8786
04/22/2021
DOI: 10.1038/s41598-021-87710-0
PMCID: PMC8062467
PMID: 33888746
url
https://doi.org/10.1038/s41598-021-87710-0View
Published (Version of record) Open Access

Abstract

Cardiovascular disease (CVD) is the most common cause of death in patients with native and post-transplant chronic kidney disease (CKD). To identify new biomarkers of vascular injury and inflammation, we analyzed the proteome of plasma and circulating extracellular vesicles (EVs) in native and post-transplant CKD patients utilizing an aptamer-based assay. Proteins of angiogenesis were significantly higher in native and post-transplant CKD patients versus healthy controls. Ingenuity pathway analysis (IPA) indicated Ephrin receptor signaling, serine biosynthesis, and transforming growth factor-β as the top pathways activated in both CKD groups. Pro-inflammatory proteins were significantly higher only in the EVs of native CKD patients. IPA indicated acute phase response signaling, insulin-like growth factor-1, tumor necrosis factor-α, and interleukin-6 pathway activation. These data indicate that pathways of angiogenesis and inflammation are activated in CKD patients' plasma and EVs, respectively. The pathways common in both native and post-transplant CKD may signal similar mechanisms of CVD.
Angiogenesis Inducing Agents - metabolism Biomarkers - metabolism Humans Inflammation - metabolism Kidney Failure, Chronic - metabolism Pilot Projects

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