Detection of recombinations between c-myc and immunoglobulin switch alpha in murine plasma cell tumors and preneoplastic lesions by polymerase chain reaction

Siegfried Janz; Jürgen Müller; John  Shaughnessy; Michael  Potter

doi:10.1073/pnas.90.15.7361

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Detection of recombinations between c-myc and immunoglobulin switch alpha in murine plasma cell tumors and preneoplastic lesions by polymerase chain reaction

Journal article

Open access

Peer reviewed

Detection of recombinations between c-myc and immunoglobulin switch alpha in murine plasma cell tumors and preneoplastic lesions by polymerase chain reaction

Siegfried Janz, Jürgen Müller, John Shaughnessy and Michael Potter

Proceedings of the National Academy of Sciences - PNAS, Vol.90(15), pp.7361-7365

08/01/1993

DOI: 10.1073/pnas.90.15.7361

PMCID: PMC47137

PMID: 8346257

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Abstract

Virtually all murine plasmacytomas carry chromosomal translocations that activate c-myc. The predominant (approximately 90%) c-myc-activating chromosomal translocation in pristane (2,6,10,14-tetramethylpentadecane)-induced plasmacytomas in BALB/c mice is a reciprocal translocation t(12;15) in which an immunoglobulin heavy-chain switch sequence is joined to the 5' region of c-myc. The most common switch region involved is S alpha. We developed a direct PCR method to screen for recombinations between c-myc and S alpha. The critical step in establishing the method was the cloning and sequencing of the 5' flank of C alpha, a region with a reduced number of switch repeats that is much more favorable for designing specific PCR primers than the highly repetitive S alpha region. In applying this PCR method, we detected translocation-specific junction fragments in transplanted (10/16, 63%) and primary (5/15, 33%) plasmacytomas. Moreover, the sensitivity of a nested version of that technique allowed us to discern rare t(12;15)s in BALB/c mice in the preneoplastic stage of plasmacytoma-genesis (8/20 mice, 40%) as early as 30 days after administration of pristane. We conclude that t(12;15) is the probable primary, if not initiating, oncogenic step in plasmacytomagenesis.

Polymerase Chain Reaction

Translocation, Genetic

Genes, Switch

Immunoglobulin alpha-Chains - genetics

Oligodeoxyribonucleotides - chemistry

Molecular Sequence Data

Plasmacytoma - genetics

Genes, myc

Precancerous Conditions - genetics

Immunoglobulin Isotypes - genetics

Genes, Immunoglobulin

Animals

Recombination, Genetic

Base Sequence

Mice

Immunoglobulin Heavy Chains - genetics

Details

Title: Subtitle: Detection of recombinations between c-myc and immunoglobulin switch alpha in murine plasma cell tumors and preneoplastic lesions by polymerase chain reaction
Creators: Siegfried Janz - Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, MD 20814
Jürgen Müller
John Shaughnessy
Michael Potter
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.90(15), pp.7361-7365
DOI: 10.1073/pnas.90.15.7361
PMID: 8346257
PMCID: PMC47137
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 08/01/1993
Academic Unit: Pathology
Record Identifier: 9984083203102771

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