Journal article
Detection of rifampin-resistant Mycobacterium tuberculosis strains by using a specialized oligonucleotide microarray
Diagnostic microbiology and infectious disease, Vol.48(1), pp.47-54
2004
DOI: 10.1016/j.diagmicrobio.2003.08.005
PMID: 14761721
Abstract
DNA microarray represents one of the major advances in diagnostic sequencing of polymerase chain reaction (PCR) products. Until now, arrays have been relatively expensive, complex to perform, and difficult to interpret, limiting their wide application in the clinical laboratory. A moderate-density oligonucleotide microarray that can rapidly identify
Mycobacterium tuberculosis rifampin-resistant strains was developed. The method is based on the detection of point mutations and other rearrangements in the
rpoB gene region determining rifampin resistance. Rifampin resistance was determined by hybridizing fluorescently labeled, amplified genetic material generated from bacterial colonies to the array. Fifty-three rifampin-resistant
M. tuberculosis and 15 rifampin-susceptible
M. tuberculosis were tested and results were concordant with those based on culture drug susceptibility testing and sequencing. Rifampin-resistant clinical isolates were detected in as little as 1.5 hours after PCR amplification with visual results. It is demonstrated that oligonucleotide microarray is an efficient, specialized technique to implement and can be used as a rapid method for detecting rifampin resistance to complement standard culture-based method.
Details
- Title: Subtitle
- Detection of rifampin-resistant Mycobacterium tuberculosis strains by using a specialized oligonucleotide microarray
- Creators
- Jun Yue - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, People's Republic of ChinaWei Shi - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, People's Republic of ChinaJingping Xie - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, People's Republic of ChinaYao Li - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, People's Republic of ChinaErliang Zeng - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, People's Republic of ChinaLi Liang - Shanghai Pulmonary Hospital, Shanghai 200433, People's Republic of ChinaHonghai Wang - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, People's Republic of China
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.48(1), pp.47-54
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.diagmicrobio.2003.08.005
- PMID
- 14761721
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 2004
- Academic Unit
- Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Biostatistics; Dental Research
- Record Identifier
- 9984065367002771
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