Determination of Rod and Cone Influence to the Early and Late Dynamic of the Pupillary Light Response

Corinne Kostic; Sylvain V Crippa; Catherine Martin; Randy H Kardon; Martin Biel; Yvan Arsenijevic; Aki Kawasaki

doi:10.1167/iovs.16-19150

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Determination of Rod and Cone Influence to the Early and Late Dynamic of the Pupillary Light Response

Journal article

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Determination of Rod and Cone Influence to the Early and Late Dynamic of the Pupillary Light Response

Corinne Kostic, Sylvain V Crippa, Catherine Martin, Randy H Kardon, Martin Biel, Yvan Arsenijevic and Aki Kawasaki

Investigative ophthalmology & visual science, Vol.57(6), pp.2501-2508

05/01/2016

DOI: 10.1167/iovs.16-19150

PMCID: PMC4868103

PMID: 27152964

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Abstract

This study aims to identify which aspects of the pupil light reflex are most influenced by rods and cones independently by analyzing pupil recordings from different mouse models of photoreceptor deficiency. One-month-old wild type (WT), rodless (Rho-/-), coneless (Cnga3-/-), or photoreceptor less (Cnga3-/-; Rho-/- or Gnat1-/-) mice were subjected to brief red and blue light stimuli of increasing intensity. To describe the initial dynamic response to light, the maximal pupillary constriction amplitudes and the derivative curve of the first 3 seconds were determined. To estimate the postillumination phase, the constriction amplitude at 9.5 seconds after light termination was related to the maximal constriction amplitude. Rho-/- mice showed decreased constriction amplitude but more prolonged pupilloconstriction to all blue and red light stimuli compared to wild type mice. Cnga3-/- mice had constriction amplitudes similar to WT however following maximal constriction, the early and rapid dilation to low intensity blue light was decreased. To high intensity blue light, the Cnga3-/- mice demonstrated marked prolongation of the pupillary constriction. Cnga3-/-; Rho-/- mice had no pupil response to red light of low and medium intensity. From specific gene defective mouse models which selectively voided the rod or cone function, we determined that mouse rod photoreceptors are highly contributing to the pupil response to blue light stimuli but also to low and medium red stimuli. We also observed that cone cells mainly drive the partial rapid dilation of the initial response to low blue light stimuli. Thus photoreceptor dysfunction can be derived from chromatic pupillometry in mouse models.

Reflex, Pupillary - physiology

Retinal Ganglion Cells - cytology

Pupil - physiology

Animals

Light

Retinal Rod Photoreceptor Cells - physiology

Rod Opsins - metabolism

Retinal Cone Photoreceptor Cells - physiology

Models, Animal

Mice

Photic Stimulation

Details

Title: Subtitle: Determination of Rod and Cone Influence to the Early and Late Dynamic of the Pupillary Light Response
Creators: Corinne Kostic - Department of Ophthalmology University Lausanne, Hôpital Ophtalmique Jules Gonin, Lausanne, Switzerland
Sylvain V Crippa - Department of Ophthalmology University Lausanne, Hôpital Ophtalmique Jules Gonin, Lausanne, Switzerland
Catherine Martin - Department of Ophthalmology University Lausanne, Hôpital Ophtalmique Jules Gonin, Lausanne, Switzerland
Randy H Kardon - Department of Ophthalmology, University of Iowa School of Medicine and Iowa City VA Center for Prevention and Treatment of Visual Loss, Iowa City, Iowa, United States
Martin Biel - Center for Integrated Protein Science Munich CiPS at the Department of Pharmacy - Center for Drug Research, Ludwig-Maximilians-Universität München, Munich, Germany
Yvan Arsenijevic - Department of Ophthalmology University Lausanne, Hôpital Ophtalmique Jules Gonin, Lausanne, Switzerland
Aki Kawasaki - Department of Ophthalmology University Lausanne, Hôpital Ophtalmique Jules Gonin, Lausanne, Switzerland
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.57(6), pp.2501-2508
DOI: 10.1167/iovs.16-19150
PMID: 27152964
PMCID: PMC4868103
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Language: English
Date published: 05/01/2016
Academic Unit: Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9983979957202771

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