Journal article
Determination of the FABP5 expression profile in skin lesions of an IMQ-induced psoriasis mouse model using flow cytometry
STAR protocols, Vol.5(2), 103018
06/21/2024
DOI: 10.1016/j.xpro.2024.103018
PMCID: PMC11026830
PMID: 38613778
Abstract
The fatty acid-binding protein 5 (FABP5) is a key player in psoriasis development. Therefore, characterizing the expression profile of FABP5 in various cell types within both layers of psoriatic skin is important. Here, we present a protocol that describes steps for an imiquimod-induced psoriasis mouse model and preparation of epidermal and dermal single-cell suspensions. We then detail procedures to detect the FABP5 expression profile in skin keratinocytes and immune cells using intracellular flow cytometry staining.
For complete details on the use and execution of this protocol, please refer to Hao et al.1
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•Instructions to induce a mouse psoriasis model using imiquimod (IMQ)•Steps for single-cell isolation from both mouse epidermis and dermis of psoriasis•Flow cytometric staining for FABP5 expression in keratinocytes and immune cells•Flow cytometric analysis of FABP5 expression in different types of skin cells
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
The fatty acid-binding protein 5 (FABP5) is a key player in psoriasis development. Therefore, characterizing the expression profile of FABP5 in various cell types within both layers of psoriatic skin is important. Here, we present a protocol that describes steps for an imiquimod-induced psoriasis mouse model and preparation of epidermal and dermal single-cell suspensions. We then detail procedures to detect the FABP5 expression profile in skin keratinocytes and immune cells using intracellular flow cytometry staining.
Details
- Title: Subtitle
- Determination of the FABP5 expression profile in skin lesions of an IMQ-induced psoriasis mouse model using flow cytometry
- Creators
- Jianyu Yu - University of IowaJiaqing Hao - University of IowaMatthew S. Yorek - University of IowaXiaochun Han - University of IowaAnthony Avellino - University of IowaXingshan Jiang - University of IowaShanshan Liu - University of IowaJinyu Wang - University of IowaBing Li - University of Iowa
- Resource Type
- Journal article
- Publication Details
- STAR protocols, Vol.5(2), 103018
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.xpro.2024.103018
- PMID
- 38613778
- PMCID
- PMC11026830
- ISSN
- 2666-1667
- eISSN
- 2666-1667
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: U01CA272424, R01CA180986, R01AI137324
- Language
- English
- Date published
- 06/21/2024
- Academic Unit
- Pathology; Surgery
- Record Identifier
- 9984616954802771
Metrics
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