Determination of the Porosity of PLGA Microparticles by Tracking Their Sedimentation Velocity Using a Flow Imaging Microscope (FlowCAM)

A S Sediq; S K D Waasdorp; M R Nejadnik; M M C van Beers; J Meulenaar; R Verrijk; W Jiskoot

doi:10.1007/s11095-017-2120-8

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Determination of the Porosity of PLGA Microparticles by Tracking Their Sedimentation Velocity Using a Flow Imaging Microscope (FlowCAM)

Journal article

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Determination of the Porosity of PLGA Microparticles by Tracking Their Sedimentation Velocity Using a Flow Imaging Microscope (FlowCAM)

A S Sediq, S K D Waasdorp, M R Nejadnik, M M C van Beers, J Meulenaar, R Verrijk and W Jiskoot

Pharmaceutical research, Vol.34(5), pp.1104-1114

05/01/2017

DOI: 10.1007/s11095-017-2120-8

PMCID: PMC5382175

PMID: 28213842

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Abstract

To investigate whether particle sedimentation velocity tracking using a flow imaging microscope (FlowCAM) can be used to determine microparticle porosity. Two different methods were explored. In the first method the sedimentation rate of microparticles was tracked in suspending media with different densities. The porosity was calculated from the average apparent density of the particles derived by inter- or extrapolation to the density of a suspending medium in which the sedimentation velocity was zero. In the second method, the microparticle size and sedimentation velocity in one suspending fluid were used to calculate the density and porosity of individual particles by using the Stokes' law of sedimentation. Polystyrene beads of different sizes were used for the development, optimization and validation of the methods. For both methods we found porosity values that were in excellent agreement with the expected values. Both methods were applied to determine the porosity of three PLGA microparticle batches with different porosities (between about 4 and 52%). With both methods we obtained microparticle porosity values similar to those obtained by mercury intrusion porosimetry. We developed two methods to determine average microparticle density and porosity by sedimentation velocity tracking, using only a few milligrams of powder.

Kinetics

Lactic Acid - chemistry

Microscopy - methods

Microspheres

Particle Size

Polyglycolic Acid - chemistry

Polylactic Acid-Polyglycolic Acid Copolymer

Porosity

Powders - chemistry

Details

Title: Subtitle: Determination of the Porosity of PLGA Microparticles by Tracking Their Sedimentation Velocity Using a Flow Imaging Microscope (FlowCAM)
Creators: A S Sediq - Centre for Human Drug Research
S K D Waasdorp - Centre for Human Drug Research
M R Nejadnik - Centre for Human Drug Research
M M C van Beers - Centre for Human Drug Research
J Meulenaar - Dr. Reddy's Laboratories (Netherlands)
R Verrijk - Dr. Reddy's Laboratories (Netherlands)
W Jiskoot - Centre for Human Drug Research
Resource Type: Journal article
Publication Details: Pharmaceutical research, Vol.34(5), pp.1104-1114
DOI: 10.1007/s11095-017-2120-8
PMID: 28213842
PMCID: PMC5382175
NLM abbreviation: Pharm Res
ISSN: 0724-8741
eISSN: 1573-904X
Grant note: DOI: 10.13039/501100003958, name: Stichting voor de Technische Wetenschappen, award: 12144
Language: English
Date published: 05/01/2017
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
Record Identifier: 9984420840902771

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