Journal article
Determining the topology of stable protein-DNA complexes
Biochemical Society transactions, Vol.41(2), pp.601-605
04/01/2013
DOI: 10.1042/BST20130004
PMID: 23514161
Abstract
Difference topology is an experimental technique that can be used to unveil the topological structure adopted by two or more DNA segments in a stable protein-DNA complex. Difference topology has also been used to detect intermediates in a reaction pathway and to investigate the role of DNA supercoiling. In the present article, we review difference topology as applied to the Mu transpososome. The tools discussed can be applied to any stable nucleoprotein complex.
Details
- Title: Subtitle
- Determining the topology of stable protein-DNA complexes
- Creators
- Isabel K Darcy - University of IowaMariel Vazquez - San Francisco State University
- Resource Type
- Journal article
- Publication Details
- Biochemical Society transactions, Vol.41(2), pp.601-605
- Publisher
- PORTLAND PRESS LTD
- DOI
- 10.1042/BST20130004
- PMID
- 23514161
- ISSN
- 0300-5127
- eISSN
- 1470-8752
- Number of pages
- 5
- Grant note
- DMS1057284 / National Science Foundation (NSF) CAREER Award NSF 0800285 / joint Division of Mathematical Sciences (DMS)-National Institute of General Medical Sciences (NIGMS) Initiative
- Language
- English
- Date published
- 04/01/2013
- Academic Unit
- Mathematics
- Record Identifier
- 9984241052302771
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