Journal article
Developing a platform system for gene delivery: amplifying virus-like particles (AVLP) as an influenza vaccine
npj vaccines, Vol.2(1), pp.32-32
11/20/2017
DOI: 10.1038/s41541-017-0031-7
PMCID: PMC5696535
PMID: 29263887
Abstract
Delivery of a gene of interest to target cells is highly desirable for translational medicine, such as gene therapy, regenerative medicine, vaccine development, and studies of gene function. Parainfluenza virus 5 (PIV5), a paramyxovirus with a negative-sense RNA genome, normally infects cells without causing obvious cytopathic effect, and it can infect many cell types. To exploit these features of PIV5, we established a system generating self-amplifying, virus-like particles (AVLP). Using enhanced green fluorescent protein (EGFP) as a reporter, AVLP encoding EGFP (AVLP-EGFP) successfully delivered and expressed the EGFP gene in primary human cells, including stem cells, airway epithelial cells, monocytes, and T cells. To demonstrate the application of this system for vaccine development, we generated AVLPs to express the HA and M1 antigens from the influenza A virus strain H5N1 (AVLP-H5 and AVLP-M1H5). Immunization of mice with AVLP-H5 and AVLP-M1H5 generated robust antibody and cellular immune responses. Vaccination with a single dose of AVLP-H5 and M1H5 completely protected mice against lethal H5N1 challenge, suggesting that the AVLP-based system is a promising platform for delivery of desirable genes.
Details
- Title: Subtitle
- Developing a platform system for gene delivery: amplifying virus-like particles (AVLP) as an influenza vaccine
- Creators
- Huiling Wei - University of GeorgiaZhenhai Chen - University of GeorgiaAndrew Elson - University of GeorgiaZhuo Li - University of GeorgiaMathew Abraham - University of GeorgiaShannon Phan - University of GeorgiaSateesh Kristhnamurthy - University of IowaPaul B. McCray - University of IowaSeth Andrews - University of GeorgiaSteve Stice - University of GeorgiaKaori Sakamoto - University of GeorgiaCheryl Jones - University of GeorgiaS. Mark Tompkins - University of GeorgiaBiao He - University of Georgia
- Resource Type
- Journal article
- Publication Details
- npj vaccines, Vol.2(1), pp.32-32
- DOI
- 10.1038/s41541-017-0031-7
- PMID
- 29263887
- PMCID
- PMC5696535
- NLM abbreviation
- NPJ Vaccines
- ISSN
- 2059-0105
- eISSN
- 2059-0105
- Publisher
- Springer Nature
- Number of pages
- 10
- Grant note
- Cystic Fibrosis Foundation; Italian Cystic Fibrosis Research Foundation P01AI060699 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) Roy J. Carver Charitable Trust NIH P30 DK-54759 / Center for Gene Therapy for Cystic Fibrosis University of Iowa In Vitro Models and Cell Culture Core P01HL051670 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) P30DK054759 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) P01 HL-51670; P01 HL-091842 / endowment Fred C. Davison Distinguished University Chair in Veterinary Medicine
- Language
- English
- Date published
- 11/20/2017
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984297332302771
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