Development and characterization of a naturally derived lung extracellular matrix hydrogel

Robert A Pouliot; Patrick A Link; Nabil S Mikhaiel; Matthew B Schneck; Michael S Valentine; Franck J Kamga Gninzeko; Joseph A Herbert; Masahiro Sakagami; Rebecca L Heise

doi:10.1002/jbm.a.35726

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Development and characterization of a naturally derived lung extracellular matrix hydrogel

Journal article

Peer reviewed

Development and characterization of a naturally derived lung extracellular matrix hydrogel

Robert A Pouliot, Patrick A Link, Nabil S Mikhaiel, Matthew B Schneck, Michael S Valentine, Franck J Kamga Gninzeko, Joseph A Herbert, Masahiro Sakagami and Rebecca L Heise

Journal of biomedical materials research. Part A, Vol.104(8), pp.1922-1935

08/01/2016

DOI: 10.1002/jbm.a.35726

PMCID: PMC7962169

PMID: 27012815

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https://www.ncbi.nlm.nih.gov/pmc/articles/7962169View

Open Access

Abstract

The complexity and rapid clearance mechanisms of lung tissue make it difficult to develop effective treatments for many chronic pathologies. We are investigating lung derived extracellular matrix (ECM) hydrogels as a novel approach for delivery of cellular therapies to the pulmonary system. The main objectives of this study include effective decellularization of porcine lung tissue, development of a hydrogel from the porcine ECM, and characterization of the material's composition, mechanical properties, and ability to support cellular growth. Our evaluation of the decellularized tissue indicated successful removal of cellular material and immunogenic remnants in the ECM. The self-assembly of the lung ECM hydrogel was rapid, reaching maximum modulus values within 3 min at 37°C. Rheological characterization showed the lung ECM hydrogel to have a concentration dependent storage modulus between 15 and 60 Pa. The purpose of this study was to evaluate our novel ECM derived hydrogel and measure its ability to support 3D culture of MSCs in vitro and in vivo delivery of MSCs. Our in vitro experiments using human mesenchymal stem cells demonstrated our novel ECM hydrogel's ability to enhance cellular attachment and viability. Our in vivo experiments demonstrated that rat MSC delivery in pre-gel solution significantly increased cell retention in the lung over 24 h in an emphysema rat model. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1922-1935, 2016.

Animals

Cell Survival - drug effects

Cell Survival - genetics

Electrophoresis, Polyacrylamide Gel

Extracellular Matrix - metabolism

Gene Expression Regulation - drug effects

Humans

Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology

Inflammation - pathology

Kinetics

Lung - metabolism

Male

Mesenchymal Stem Cells - drug effects

Mesenchymal Stem Cells - metabolism

Pancreatic Elastase

Rats, Sprague-Dawley

Rheology

Sus scrofa

Details

Title: Subtitle: Development and characterization of a naturally derived lung extracellular matrix hydrogel
Creators: Robert A Pouliot - Virginia Commonwealth University
Patrick A Link - Virginia Commonwealth University
Nabil S Mikhaiel - Virginia Commonwealth University
Matthew B Schneck - Virginia Commonwealth University
Michael S Valentine - Virginia Commonwealth University
Franck J Kamga Gninzeko - Virginia Commonwealth University
Joseph A Herbert - Virginia Commonwealth University
Masahiro Sakagami - Virginia Commonwealth University
Rebecca L Heise - Virginia Commonwealth University
Resource Type: Journal article
Publication Details: Journal of biomedical materials research. Part A, Vol.104(8), pp.1922-1935
DOI: 10.1002/jbm.a.35726
PMID: 27012815
PMCID: PMC7962169
NLM abbreviation: J Biomed Mater Res A
ISSN: 1549-3296
eISSN: 1552-4965
Grant note: P30 NS047463 / NINDS NIH HHS P30 CA016059 / NCI NIH HHS
Language: English
Date published: 08/01/2016
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center
Record Identifier: 9984948144502771

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