Journal article
Development of Brainstem 5-HT1A Receptor Binding Sites in Serotonin-Deficient Mice
Journal of neurochemistry, Vol.126(6), pp.749-757
09/2013
DOI: 10.1111/jnc.12311
PMCID: PMC3987866
PMID: 23692315
Abstract
The sudden infant death syndrome (SIDS) is associated with a reduction in brainstem serotonin (5-hydroxytryptamine, 5-HT) and 5-HT
1A
receptor binding, yet it is unknown if and how these findings are linked. In this study, we used quantitative tissue autoradiography to determine if postnatal development of brainstem 5-HT
1A
receptors is altered in two mouse models where the development of 5-HT neurons is defective, the
Lmx1b
f/f/p
, and the
Pet-1
-/-
mouse. 5-HT
1A
receptor agonist-binding sites were examined in both 5-HT-source nuclei (autoreceptors) and in sites that receive 5-HT innervation (heteroreceptors). In control mice between postnatal day (P) 3 and 10, 5-HT
1A
receptor binding increased in several brainstem sites; by P25, there were region-specific increases and decreases, refining the overall binding pattern. In the
Lmx1b
f/f/p
and
Pet-1
-/-
mice, 5-HT
1A
-autoreceptor binding was significantly lower than in control mice at P3, and remained low at P10 and P25. In contrast, 5-HT
1A
heteroreceptor levels were comparable between control and 5-HT-deficient mice. These data define the postnatal development of 5-HT
1A
receptor binding in the mouse brainstem. Furthermore the data suggest that 5-HT
1A
heteroreceptor deficits detected in SIDS are not a direct consequence of a 5-HT neuron dysfunction nor reduced brain 5-HT levels.
Details
- Title: Subtitle
- Development of Brainstem 5-HT1A Receptor Binding Sites in Serotonin-Deficient Mice
- Creators
- Caitlin A Massey - Department of Anesthesiology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115Gloria Kim - Department of Anesthesiology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115Andrea E Corcoran - Geisel School of Medicine at Dartmouth, Lebanon, NH, USA 03756Robin L Haynes - Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115David S Paterson - Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115Kevin J Cummings - Geisel School of Medicine at Dartmouth, Lebanon, NH, USA 03756Susan M Dymecki - Department of Genetics, Harvard Medical School, Boston, MA 02115George B Richerson - Department of Neurology, University of Iowa, Iowa City, IA 52242Eugene E Nattie - Geisel School of Medicine at Dartmouth, Lebanon, NH, USA 03756Hannah C Kinney - Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115Kathryn G Commons - Department of Anesthesiology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115
- Resource Type
- Journal article
- Publication Details
- Journal of neurochemistry, Vol.126(6), pp.749-757
- DOI
- 10.1111/jnc.12311
- PMID
- 23692315
- PMCID
- PMC3987866
- NLM abbreviation
- J Neurochem
- ISSN
- 0022-3042
- eISSN
- 1471-4159
- Grant note
- name: NIH, award: PO1 HD-036379
- Language
- English
- Date published
- 09/2013
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Neurosurgery
- Record Identifier
- 9984020765102771
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