Development of In Vivo Impedance Spectroscopy Techniques for Measurement of Micropore Formation Following Microneedle Insertion

Nicole K Brogden; Priyanka Ghosh; Lucia Hardi; Leslie J Crofford; Audra L Stinchcomb

doi:10.1002/jps.23544

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Development of In Vivo Impedance Spectroscopy Techniques for Measurement of Micropore Formation Following Microneedle Insertion

Journal article

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Development of In Vivo Impedance Spectroscopy Techniques for Measurement of Micropore Formation Following Microneedle Insertion

Nicole K Brogden, Priyanka Ghosh, Lucia Hardi, Leslie J Crofford and Audra L Stinchcomb

Journal of pharmaceutical sciences, Vol.102(6), pp.1948-1956

06/2013

DOI: 10.1002/jps.23544

PMCID: PMC3678392

PMID: 23589356

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https://www.ncbi.nlm.nih.gov/pmc/articles/3678392View

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Abstract

Microneedles (MNs) provide a minimally invasive means to enhance skin permeability by creating micron-scale channels (micropores) that provide a drug delivery pathway. Adequate formation of the micropores is critical to the success of this unique drug delivery technique. The objective of the current work was to develop sensitive and reproducible impedance spectroscopy techniques to monitor micropore formation in animal models and human subjects. Hairless guinea pigs, a Yucatan miniature pig, and human volunteers were treated with 100 MN insertions per site following an overnight prehydration period. Repeated measurements were made pre- and post-MN treatment using dry and gel Ag/AgCl electrodes applied with light verses direct pressure to hold the electrode to the skin surface. Impedance measurements dropped significantly post-MN application at all sites (p<0.05, irrespective of electrode type or gel application), confirming micropore formation. In the Yucatan pig and human subjects, gel electrodes with direct pressure yielded the lowest variability (demonstrated by lower %relative standard deviation), whereas dry electrodes with direct pressure were superior in the guinea pigs. These studies confirm that impedance measurements are suitable for use in both clinical and animal research environments to monitor the formation of new micropores that will allow for drug delivery through the impermeable skin layers.

microneedle

impedance spectroscopy

percutaneous

transdermal

active transport

micropore

permeability

skin

human

Details

Title: Subtitle: Development of In Vivo Impedance Spectroscopy Techniques for Measurement of Micropore Formation Following Microneedle Insertion
Creators: Nicole K Brogden - University of Kentucky College of Pharmacy, Department of Pharmaceutical Sciences Lexington Kentucky
Priyanka Ghosh - University of Kentucky College of Pharmacy, Department of Pharmaceutical Sciences Lexington Kentucky
Lucia Hardi - University of Kentucky, Department of Internal Medicine Lexington Kentucky
Leslie J Crofford - University of Kentucky, Department of Internal Medicine Lexington Kentucky
Audra L Stinchcomb - University of Kentucky College of Pharmacy, Department of Pharmaceutical Sciences Lexington Kentucky
Resource Type: Journal article
Publication Details: Journal of pharmaceutical sciences, Vol.102(6), pp.1948-1956
DOI: 10.1002/jps.23544
PMID: 23589356
PMCID: PMC3678392
NLM abbreviation: J Pharm Sci
ISSN: 0022-3549
eISSN: 1520-6017
Publisher: Elsevier Inc
Language: English
Date published: 06/2013
Academic Unit: Dermatology; Pharmaceutical Sciences and Experimental Therapeutics; Physical Therapy and Rehabilitation Science
Record Identifier: 9984025446202771

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