Development of Shortened Enrichment Methods for Detection of Salmonella Typhimurium Spiked in Milk

Kevin Tsai; Matthew W Nonnenmann; Diane Rohlman; Kelly K. Baker

doi:10.1021/acsfoodscitech.2c00310

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Development of Shortened Enrichment Methods for Detection of Salmonella Typhimurium Spiked in Milk

Journal article

Open access

Peer reviewed

Development of Shortened Enrichment Methods for Detection of Salmonella Typhimurium Spiked in Milk

Kevin Tsai, Matthew W Nonnenmann, Diane Rohlman and Kelly K. Baker

ACS food science & technology, Vol.3(5), pp.831-837

04/21/2023

DOI: 10.1021/acsfoodscitech.2c00310

PMCID: PMC10204049

PMID: 37228389

Appears in UI Libraries Support Open Access

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https://doi.org/10.1021/acsfoodscitech.2c00310View

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Abstract

Rapid and accurate testing of pathogenic Salmonella enterica in dairy products could reduce the risk of exposure to the bacterial pathogens for consumers. This study aimed to reduce the assessment time needed for enteric bacteria recovery and quantification in food using the natural growth properties of Salmonella enterica Typhimurium (S. Typhimurium) in cow’s milk and efficiently using rapid PCR methods. Over 5 h of 37 °C enrichment, culture and PCR methods measured increases in the non-heat-treated S. Typhimurium concentration at similar rates, with an average increase of 2.7 log10 CFU/mL between the start of enrichment and 5 h. In contrast, no bacteria were recovered by culture after S. Typhimurium in milk received heat treatment, and the number of gene copies of heat-treated Salmonella detected by PCR did not increase with the enrichment time. Thus, comparing culture and PCR data over just 5 h of enrichment time can detect and differentiate between replicating bacteria and dead bacteria.

Food Safety

Salmonella Typhimurium

milk

enrichment

real-time PCR

UIOWA OA Agreement

Details

Title: Subtitle: Development of Shortened Enrichment Methods for Detection of Salmonella Typhimurium Spiked in Milk
Creators: Kevin Tsai - University of Iowa
Matthew W Nonnenmann - University of Nebraska Medical Center
Diane Rohlman - University of Iowa
Kelly K. Baker - University of Iowa
Resource Type: Journal article
Publication Details: ACS food science & technology, Vol.3(5), pp.831-837
DOI: 10.1021/acsfoodscitech.2c00310
PMID: 37228389
PMCID: PMC10204049
NLM abbreviation: ACS Food Sci Technol
ISSN: 2692-1944
eISSN: 2692-1944
Publisher: American Chemical Society
Grant note: DOI: 10.13039/100000061, name: Fogarty International Center, award: R01 TW011795; DOI: 10.13039/100008893, name: University of Iowa
Language: English
Date published: 04/21/2023
Academic Unit: Public Health Administration; Occupational and Environmental Health; International Programs; Epidemiology; Injury Prevention Research Center; Internal Medicine
Record Identifier: 9984410785202771

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