Journal article
Development of a bilayer ring system for achieving high strain in commercial rheometers
Journal of biomechanics, Vol.48(12), pp.3512-3516
09/18/2015
DOI: 10.1016/j.jbiomech.2015.07.011
PMCID: PMC4592812
PMID: 26228712
Abstract
Mechanical stimulation of cell cultures has been shown be an effective means of enhancing ECM production. ECM produced from vocal fold fibroblast cultures has the potential for therapeutic use for vocal fold repair. However, current bioreactor designs generally fail to produce physiological relevant frequency and strain values. Here we present an approach for using commercial oscillatory rheometers and an elastic ring bilayer system to produce physiologically relevant strain values at frequencies in the range of 20–100Hz. We demonstrate the ability to target specific strain and frequency values by manipulating system parameters, and also show that it is possible to maintain high oscillatory strains for extended periods of time. Such a system could be used to mechanically stimulate cell cultures contained within gel carrier systems and has the potential to be extended to other applications requiring high strains at low frequencies.
Details
- Title: Subtitle
- Development of a bilayer ring system for achieving high strain in commercial rheometers
- Creators
- Michael B. Christensen - University of UtahJeffrey C. Wolchok - University of Arkansas at FayettevilleSarah A. Klemuk - University of IowaIngo R. Titze - University of Utah
- Resource Type
- Journal article
- Publication Details
- Journal of biomechanics, Vol.48(12), pp.3512-3516
- DOI
- 10.1016/j.jbiomech.2015.07.011
- PMID
- 26228712
- PMCID
- PMC4592812
- NLM abbreviation
- J Biomech
- ISSN
- 0021-9290
- eISSN
- 1873-2380
- Publisher
- Elsevier Ltd
- Number of pages
- 5
- Language
- English
- Date published
- 09/18/2015
- Academic Unit
- School of Music; Communication Sciences and Disorders
- Record Identifier
- 9984719574102771
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