Journal article
Development of a highly sensitive and specific carboxy-terminal human pancreastatin assay to monitor neuroendocrine tumor behavior
Pancreas, Vol.39(5), pp.611-616
07/2010
DOI: 10.1097/MPA.0b013e3181c68d7a
PMID: 20124939
Abstract
Pancreastatin is a fragment of the chromogranin A (CgA) molecule. Existing pancreastatin assays, which depend on antibodies that cross-react in varying percents with the larger prohormone, may lack sensitivity and specificity to detect small changes in neuroendocrine tumor volume.
We developed a highly specific, sensitive pancreastatin assay. The antibody used recognizes the carboxyl terminal of the peptide hormone and was raised against a 17-amino acid porcine pancreastatin fragment with high homology with the carboxy-terminal amino acids 286-301 of the human CgA.
Our assay measures more than 95% of circulating pancreastatin levels; has little or no cross-reactivity with CgA, even at plasma concentrations of 1000 ng/mL; and can detect pancreastatin levels of 17 pg/mL. Interassay reproducibility for the pancreastatin radioimmunoassay was determined from results of 3 quality control pools in 15 consecutive assays. Coefficients of variation for low, medium, and high pancreastatin levels were less than 20%. The sensitivity of serial pancreastatin assays to detect early liver tumor activity was demonstrated in 2 patients with slowly progressive neuroendocrine tumors and in patients undergoing surgical cytoreduction.
This highly specific, sensitive pancreastatin assay can detect small changes in liver tumor progression and is up to 100-fold more sensitive and specific than CgA assays in the United States.
Details
- Title: Subtitle
- Development of a highly sensitive and specific carboxy-terminal human pancreastatin assay to monitor neuroendocrine tumor behavior
- Creators
- Thomas M O'Dorisio - Department of Internal Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA, USA. Thomas-odorisio@uiowa.eduSiegfried R KrutzikEugene A WolteringErika LindholmSaju JosephAbby E GandolfiYi-Zarn WangJ Phillip BoudreauxAaron I VinikVay Liang W GoJames R HoweThor HalfdanarsonM Sue O'DorisioGregg Mamikunian
- Resource Type
- Journal article
- Publication Details
- Pancreas, Vol.39(5), pp.611-616
- Publisher
- United States
- DOI
- 10.1097/MPA.0b013e3181c68d7a
- PMID
- 20124939
- ISSN
- 0885-3177
- eISSN
- 1536-4828
- Language
- English
- Date published
- 07/2010
- Academic Unit
- Stead Family Department of Pediatrics; Surgery; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984051592502771
Metrics
34 Record Views