Journal article
Development of a novel tissue‐mimicking color calibration slide for digital microscopy
Color research and application, Vol.43(2), pp.184-197
04/2018
DOI: 10.1002/col.22187
Abstract
Digital microscopy produces high resolution digital images of pathology slides. Because no acceptable and effective control of color reproduction exists in this domain, there is significant variability in color reproduction of whole slide images. Guidance from international bodies and regulators highlights the need for color standardization. To address this issue, we systematically measured and analyzed the spectra of histopathological stains. This information was used to design a unique color calibration slide utilizing real stains and a tissue‐like substrate, which can be stained to produce the same spectral response as tissue. By closely mimicking the colors in stained tissue, our target can provide more accurate color representation than film‐based targets, whilst avoiding the known limitations of using actual tissue. The application of the color calibration slide in the clinical setting was assessed by conducting a pilot user‐evaluation experiment with promising results. With the imminent integration of digital pathology into the routine work of the diagnostic pathologist, it is hoped that this color calibration slide will help provide a universal color standard for digital microscopy thereby ensuring better and safer healthcare delivery.
Details
- Title: Subtitle
- Development of a novel tissue‐mimicking color calibration slide for digital microscopy
- Creators
- Emily L Clarke - Leeds Teaching Hospitals NHS TrustCraig Revie - FFEI LimitedDavid Brettle - Leeds Teaching Hospitals NHS TrustMichael Shires - University of LeedsPeter Jackson - Leeds Teaching Hospitals NHS TrustRavinder Cochrane - FFEI LimitedRobert Wilson - FFEI LimitedClaudia Mello‐Thoms - University of SydneyDarren Treanor - Centre for Medical Image Visualisation, Linkoping University
- Resource Type
- Journal article
- Publication Details
- Color research and application, Vol.43(2), pp.184-197
- DOI
- 10.1002/col.22187
- ISSN
- 0361-2317
- eISSN
- 1520-6378
- Number of pages
- 14
- Grant note
- UK Technology Strategy Board (720200 2012)
- Language
- English
- Date published
- 04/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology
- Record Identifier
- 9984051540402771
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