Development of a poly (lactic-co-glycolic acid) particle vaccine to protect against house dust mite induced allergy

Vijaya B Joshi; Andrea Adamcakova-Dodd; Xuefang Jing; Amaraporn Wongrakpanich; Katherine N Gibson-Corley; Peter S Thorne; Aliasger K Salem

doi:10.1208/s12248-014-9624-5

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Development of a poly (lactic-co-glycolic acid) particle vaccine to protect against house dust mite induced allergy

Journal article

Open access

Peer reviewed

Development of a poly (lactic-co-glycolic acid) particle vaccine to protect against house dust mite induced allergy

Vijaya B Joshi, Andrea Adamcakova-Dodd, Xuefang Jing, Amaraporn Wongrakpanich, Katherine N Gibson-Corley, Peter S Thorne and Aliasger K Salem

The AAPS journal, Vol.16(5), pp.975-985

09/2014

DOI: 10.1208/s12248-014-9624-5

PMCID: PMC4147059

PMID: 24981892

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Abstract

Poly(lactic-co-glycolic acid) (PLGA) particles carrying antigen and adjuvant is a promising vaccine system which has been shown to stimulate systemic antigen-specific immune responses. In this study, we investigated the relationship of (i) the sizes of PLGA particle and (ii) the presence of cytosine-phosphate-guanine motifs (CpG), with the extent and type of immune response stimulated against Dermatophagoides pteronyssinus-2 (Der p2) antigen. Different sizes of PLGA particles encapsulating CpG were prepared using a double emulsion solvent evaporation method. Mice were vaccinated with Der p2 and different sizes of empty or CpG-loaded PLGA particles. Vaccinated mice were exposed to daily intranasal instillation of Der p2 for 10 days followed by euthanization to estimate leukocyte accumulation in bronchoalveolar lavage (BAL) fluids, antibody profiles, and airway hyperresponsiveness. PLGA particles showed a size-dependent decrease in the proportion of eosinophils found in BAL fluids. Mice vaccinated with the Der p2 coated on 9-μm-sized empty PLGA particles showed increased levels of IgE and IgG1 antibodies as well as increased airway hyperresponsiveness. All sizes of PLGA particles encapsulating CpG prevented airway hyperresponsiveness after Der p2 exposures. Inflammatory responses to Der p2 exposure were significantly reduced when smaller PLGA particles were used for vaccination. In addition, encapsulating CpG in PLGA particles increased IgG2a secretion. This study shows that the size of PLGA particles used for vaccination plays a major role in the prevention of house dust mite-induced allergy and that incorporation of CpG into the PLGA particles preferentially develops a Th1-type immune response.

Adjuvants, Immunologic - pharmacology

Bronchial Hyperreactivity - immunology

Immunoglobulin G - blood

Vaccination

Male

Antigens, Dermatophagoides - pharmacology

Th1 Cells - immunology

Immunoglobulin E - blood

Bronchial Hyperreactivity - blood

Drug Carriers

Antigens, Dermatophagoides - immunology

Vaccines - pharmacology

Vaccines - chemistry

Adjuvants, Immunologic - chemistry

Bronchial Hyperreactivity - physiopathology

Respiratory Hypersensitivity - prevention & control

Respiratory Hypersensitivity - physiopathology

Disease Models, Animal

Th1 Cells - drug effects

Lactic Acid - chemistry

Solubility

Vaccines - immunology

Technology, Pharmaceutical - methods

Lung - physiopathology

Bronchial Hyperreactivity - prevention & control

Respiratory Hypersensitivity - immunology

Chemistry, Pharmaceutical

Mice, Inbred C3H

Particle Size

Respiratory Hypersensitivity - blood

Animals

Lung - drug effects

Polyglycolic Acid - chemistry

CpG Islands

Mice

Kinetics

Antigens, Dermatophagoides - chemistry

Lung - immunology

Pyroglyphidae - immunology

Details

Title: Subtitle: Development of a poly (lactic-co-glycolic acid) particle vaccine to protect against house dust mite induced allergy
Creators: Vijaya B Joshi - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, Iowa, 52242, USA
Andrea Adamcakova-Dodd
Xuefang Jing
Amaraporn Wongrakpanich
Katherine N Gibson-Corley
Peter S Thorne
Aliasger K Salem
Resource Type: Journal article
Publication Details: The AAPS journal, Vol.16(5), pp.975-985
DOI: 10.1208/s12248-014-9624-5
PMID: 24981892
PMCID: PMC4147059
NLM abbreviation: AAPS J
ISSN: 1550-7416
eISSN: 1550-7416
Publisher: United States
Grant note: P30 ES005605 / NIEHS NIH HHS 1R21CA13345-01 / NCI NIH HHS 1R21CA128414-01A2/UI / NCI NIH HHS R21 CA128414 / NCI NIH HHS P30 DK054759 / NIDDK NIH HHS
Language: English
Date published: 09/2014
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Civil and Environmental Engineering; Occupational and Environmental Health; Stead Family Department of Pediatrics; Pathology; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Ophthalmology and Visual Sciences
Record Identifier: 9983985962902771

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