Journal article
Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping
Human genetics, Vol.91(3), pp.199-204
04/1993
DOI: 10.1007/BF00218256
PMID: 8478002
Abstract
We sequenced the alphoid centromere probe pα10RP8 (D10Z1), aligned it to three published consensus sequences, and developed a sequence-tagged site (STS), sJRH-2, based upon oligonucleotide primers having two 3′ mismatches with these consensus sequences. Polymerase chain reaction (PCR) amplification using genomic DNA from a somatic cell hybrid panel representing all human chromosomes demonstrated amplification from only those cell lines containing chromosome 10. Fluorescence in situ hybridization of the amplified product demonstrated intense and specific hybridization of the PCR product to 10p11.1-q11.1. A human genomic yeast artificial chromosome (YAC) library was screened using the sJRH-2 PCR assay, and five clones were identified. Sequence analysis of one chimeric clone (consisting of DNA segments derived from chromosomes 5p and 10cen) confirmed specificity of the STS for the centromere of chromosome 10. sJRH-2 provides a convenient cytogenetic marker for chromosome 10, which will also be useful for physical mapping of the pericentromeric region of chromosome 10, a region that harbors the gene(s) for three forms of multiple endocrine neoplasia (types 2A, 2B, and familial medullary thyroid carcinoma). The GenBank accession number for the pα10RP8 sequence is X63622. © 1993 Springer-Verlag.
Details
- Title: Subtitle
- Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping
- Creators
- James R. Howe - Washington University in St. LouisTerry C. Lairmore - Washington University in St. LouisRosalie Veile - Washington University in St. LouisShenshen Dou - Washington University in St. LouisSamuel A. Wells - Washington University in St. LouisHelen Donis-Keller - Washington University in St. Louis
- Resource Type
- Journal article
- Publication Details
- Human genetics, Vol.91(3), pp.199-204
- DOI
- 10.1007/BF00218256
- PMID
- 8478002
- ISSN
- 0340-6717
- eISSN
- 1432-1203
- Language
- English
- Date published
- 04/1993
- Academic Unit
- Surgery
- Record Identifier
- 9984322946302771
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