Development of an ELISA to Detect Anti-BP180 Autoantibodies in Bullous Pemphigoid and Herpes Gestationis

George J Giudice; Kendell C Wilske; Grant J Anhalt; Janet A Fairley; Ann F Taylor; Daryl J Emery; Raymond G Hoffman; Luis A Diaz

doi:10.1111/1523-1747.ep12382738

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Development of an ELISA to Detect Anti-BP180 Autoantibodies in Bullous Pemphigoid and Herpes Gestationis

Journal article

Peer reviewed

Development of an ELISA to Detect Anti-BP180 Autoantibodies in Bullous Pemphigoid and Herpes Gestationis

George J Giudice, Kendell C Wilske, Grant J Anhalt, Janet A Fairley, Ann F Taylor, Daryl J Emery, Raymond G Hoffman and Luis A Diaz

Journal of investigative dermatology, Vol.102(6), pp.878-881

06/1994

DOI: 10.1111/1523-1747.ep12382738

PMID: 7516396

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Abstract

Autoantibodies associated with the subepidermal blistering disorders bullous pemphigoid and herpes gestationis react with a 180-kD transmembrane hemidesmosomal protein, designated BP180. The BP180 ectodomain is composed of a series of interrupted collagen triple helical domains. Located on one of the noncollagenous extracellular segments of this protein is an immunodominant epitope, designated MCW-1, recognized by patient autoantibodies. In this investigation we have developed an enzyme-linked immunosorbent assay system to detect antibody reactivity against the MCW-1 epitope with the use of a bacterial fusion protein containing the BP180 autoantibody-reactive site. The following sera were assayed for reactivity with this recombinant protein: bullous pemphigoid (n = 62), herpes gestationis (n = 28), endemic pemphigus foliaceus (n = 17), lupus erythematosus (n = 15), and normal human sera (n = 22). This enzyme-linked immunosorbent assay – based protocol was shown to be highly specific (98.3%) in detecting autoantibody activity in bullous pemphigoid and herpes gestationis patients. Fifty-three percent of bullous pemphigoid and 71% of herpes gestationis sera, but none of the control sera, yielded positive results in this assay. Of the patient sera that were known to react with full-length BP180, almost all showed reactivity with the MCW-1 antigenic site of this protein. Autoantibodies detected in this assay were predominantly of the immunoglobulin G class. The results presented here lend support to the hypothesis that this well-defined antigen/antibody system may be relevant in pathogenesis.

skin

collagen

basement membrane

autoimmunity

hemidesmosome

Details

Title: Subtitle: Development of an ELISA to Detect Anti-BP180 Autoantibodies in Bullous Pemphigoid and Herpes Gestationis
Creators: George J Giudice - Departments of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin, U.S.A
Kendell C Wilske - Departments of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin, U.S.A
Grant J Anhalt - Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A
Janet A Fairley - Departments of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin, U.S.A
Ann F Taylor - Departments of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin, U.S.A
Daryl J Emery - Departments of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin, U.S.A
Raymond G Hoffman - Departments of Biostatistics and Clinical Epidemiology, Medical College of Wisconsin, Milwaukee, Wisconsin
Luis A Diaz - Departments of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin, U.S.A
Resource Type: Journal article
Publication Details: Journal of investigative dermatology, Vol.102(6), pp.878-881
DOI: 10.1111/1523-1747.ep12382738
PMID: 7516396
NLM abbreviation: J Invest Dermatol
ISSN: 0022-202X
eISSN: 1523-1747
Publisher: Elsevier Inc
Language: English
Date published: 06/1994
Academic Unit: Dermatology
Record Identifier: 9984025560202771

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