Journal article
Development of an In Vitro Candida albicans Oral Infection Model
International journal of oral biology, Vol.22(4), pp.249-253
12/31/1997
Abstract
Objectives: The purpose of the present study was to develop an in vitro model for studies of Candida albicans oral infections. Design: Sheets of porcine buccal epithelium were cut at a thickness of 300 pm and glued with cyanoacrylate to a plastic backing. Circular 1-cm-diameter disks of the plastic-backed epithelium were cut, and incubated with Candida albicans. At various incubation times, numbers and appearance of Candida albicans on the tissue disks were assessed. Materials: Porcine tissue was obtained at a slaughterhouse. Candida selective Chromager plates were purchased from Hardy Diagnostics of Santa Maria, CA. Other materials were from Sigma Chemical Company, St. Louis, MO. Methods: Candida albicans were cultured in Sabouraud Dextrose Broth at 37°C, overnight. Tissue disks were incubated with standardized suspensions. At time of harvest, some disks were fixed for examination by scanning electron microscopy, while others were homogenized and spiral-plated onto Candida-selective agar. Results: At three hours, there were 2.1 x 105 adherent yeast per disk. By 24 hours of incubation, there were noticeable hyphae formation, and by 48 hours hyphae predominated and tissue invasion was evident. Conclusions: The porcine buccal epithelial disk provides an in vitro model for oral Candida albicans infections in which adherence, growth, and the bud to hyphae transition and tissue invasion process may be investigated.
Details
- Title: Subtitle
- Development of an In Vitro Candida albicans Oral Infection Model
- Creators
- David R. DrakeTrace LundCynthia L. KleineggerPhilip W. Wertz
- Resource Type
- Journal article
- Publication Details
- International journal of oral biology, Vol.22(4), pp.249-253
- Publisher
- 대한구강생물학회
- ISSN
- 1226-7155
- eISSN
- 2287-6618
- Number of pages
- 5
- Language
- English
- Date published
- 12/31/1997
- Academic Unit
- Dental Research; Oral Pathology, Radiology and Medicine; Endodontics
- Record Identifier
- 9984367599302771
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