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Development of cystic fibrosis and noncystic fibrosis airway cell lines
Journal article   Open access   Peer reviewed

Development of cystic fibrosis and noncystic fibrosis airway cell lines

Joseph Zabner, Phil Karp, Michael Seiler, Stacia L Phillips, Calista J Mitchell, Mimi Saavedra, Michael Welsh and Aloysius J Klingelhutz
American journal of physiology. Lung cellular and molecular physiology, Vol.284(5), pp.L844-L854
05/01/2003
DOI: 10.1152/ajplung.00355.2002
PMID: 12676769
url
https://doi.org/10.1152/ajplung.00355.2002View
Published (Version of record) Open Access

Abstract

In this study, we utilized the reverse transcriptase component of telomerase, hTERT, and human papillomavirus type 16 (HPV-16) E6 and E7 genes to transform normal and cystic fibrosis (CF) human airway epithelial (HAE) cells. One cell line, designated NuLi-1 (normal lung, University of Iowa), was derived from HAE of normal genotype; three cell lines, designated CuFi (cystic fibrosis, University of Iowa)-1, CuFi-3, and CuFi-4, were derived from HAE of various CF genotypes. When grown at the air-liquid interface, the cell lines were capable of forming polarized differentiated epithelia that exhibited transepithelial resistance and maintained the ion channel physiology expected for the genotypes. The CF transmembrane conductance regulator defect in the CuFi cell lines could be corrected by infecting from the basolateral surface using adenoviral vectors. Using nuclear factor-κB promoter reporter constructs, we also demonstrated that the NuLi and CuFi cell lines retained nuclear factor-κB responses to lipopolysaccharide. These cell lines should therefore be useful as models for studying ion physiology, therapeutic intervention for CF, and innate immunity.

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