Journal article
Development of human alveolar epithelial cell models to study distal lung biology and disease
iScience, Vol.25(2), pp.103780-103780
02/18/2022
DOI: 10.1016/j.isci.2022.103780
PMCID: PMC8829779
PMID: 35169685
Abstract
Many acute and chronic diseases affect the distal lung alveoli. Alveolar epithelial cell (AEC) lines are needed to better model these diseases. We used de-identified human remnant transplant lungs to develop a method to establish AEC lines. The lines grow well in 2-dimensional (2D) culture as epithelial monolayers expressing lung progenitor markers. In 3-dimensional (3D) culture with fibroblasts, Matrigel, and specific media conditions, the cells form alveolar-like organoids expressing mature AEC markers including aquaporin 5 (AQP5), G-protein-coupled receptor class C group 5 member A (GPRC5A), and surface marker HTII280. Single-cell RNA sequencing of an AEC line in 2D versus 3D culture revealed increased cellular heterogeneity and induction of cytokine and lipoprotein signaling in 3D organoids. Our approach yields lung progenitor lines that retain the ability to differentiate along the alveolar cell lineage despite long-term expansion and provides a valuable system to model and study the distal lung in vitro.
Details
- Title: Subtitle
- Development of human alveolar epithelial cell models to study distal lung biology and disease
- Creators
- Evelyn Tran - University of Southern CaliforniaTuo Shi - University of Southern CaliforniaXiuwen Li - University of Southern CaliforniaAdnan Y. Chowdhury - University of Southern CaliforniaDu Jiang - University of Southern CaliforniaYixin Liu - University of Southern CaliforniaHongjun Wang - University of Southern CaliforniaChunli Yan - University of Southern CaliforniaWilliam D. Wallace - University of Southern CaliforniaRong Lu - University of Southern CaliforniaAmy L. Ryan - University of Southern CaliforniaCrystal N. Marconett - University of Southern CaliforniaBeiyun Zhou - University of Southern CaliforniaZea Borok - University of Southern CaliforniaIte A. Offringa - University of Southern California
- Resource Type
- Journal article
- Publication Details
- iScience, Vol.25(2), pp.103780-103780
- DOI
- 10.1016/j.isci.2022.103780
- PMID
- 35169685
- PMCID
- PMC8829779
- NLM abbreviation
- iScience
- ISSN
- 2589-0042
- eISSN
- 2589-0042
- Publisher
- Elsevier
- Number of pages
- 32
- Grant note
- Rose Hills Foundation Hastings Foundation Hearst Foundation, Inc. Fellowship BAPP-15-121814 / California Community Foundation 9221013100 / Keck Foundation; W.M. Keck Foundation P30CA014089 / Norris Comprehensive Cancer Center core grant from the National Cancer Institute RSG-20-135-01 / American Cancer Society USC Office of Research California Institute for Regenerative Medicine Training grant R01 HL114094; U54 CA233465; R35 HL135747; R01 HL139828; R00 HL113104; R01 HL135292; R01 HL138225; R35 HL150826 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA USC Libraries
- Language
- English
- Date published
- 02/18/2022
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284323302771