Journal article
Development, validation, and potential applications of biotinylated red blood cells for posttransfusion kinetics and other physiological studies: evidenced‐based analysis and recommendations
Transfusion (Philadelphia, Pa.), Vol.58(8), pp.2068-2081
08/2018
DOI: 10.1111/trf.14647
PMCID: PMC6160343
PMID: 29770455
Abstract
The current reference method in the United States for measuring in vivo population red blood cell (RBC) kinetics utilizes chromium‐51 (51Cr) RBC labeling for determining RBC volume, 24‐hour posttransfusion RBC recovery, and long‐term RBC survival. Here we provide evidence supporting adoption of a method for kinetics that uses the biotin‐labeled RBCs (BioRBCs) as a superior, versatile method for both regulatory and investigational purposes. RBC kinetic analysis using BioRBCs has important methodologic, analytical, and safety advantages over 51Cr‐labeled RBCs. We critically review recent advances in labeling human RBCs at multiple and progressively lower biotin label densities for concurrent, accurate, and sensitive determination of both autologous and allogeneic RBC population kinetics. BioRBC methods valid for RBC kinetic studies, including successful variations used by the authors, are presented along with pharmacokinetic modeling approaches for the accurate determination of RBC pharmacokinetic variables in health and disease. The advantages and limitations of the BioRBC method—including its capability of determining multiple BioRBC densities simultaneously in the same individual throughout the entire RBC life span—are presented and compared with the 51Cr method. Finally, potential applications and limitations of kinetic BioRBC determinations are discussed.
Details
- Title: Subtitle
- Development, validation, and potential applications of biotinylated red blood cells for posttransfusion kinetics and other physiological studies: evidenced‐based analysis and recommendations
- Creators
- Donald M. Mock - University of Arkansas for Medical SciencesDemet Nalbant - University of IowaSvetlana V. Kyosseva - University of Arkansas for Medical SciencesRobert L. Schmidt - University of IowaGuohua An - University of IowaNell I. Matthews - University of Arkansas for Medical SciencesAlexander P.J. Vlaar - Amsterdam UMC Location University of AmsterdamRobin van Bruggen - SanquinDirk de Korte - SanquinRonald G. Strauss - University of IowaJosé A. Cancelas - University of CincinnatiRobert S. Franco - University of Cincinnati Medical CenterPeter Veng-Pedersen - University of IowaJohn A. Widness - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Transfusion (Philadelphia, Pa.), Vol.58(8), pp.2068-2081
- DOI
- 10.1111/trf.14647
- PMID
- 29770455
- PMCID
- PMC6160343
- NLM abbreviation
- Transfusion
- ISSN
- 0041-1132
- eISSN
- 1537-2995
- Number of pages
- 14
- Grant note
- The United States Public Health Service National Institutes of Health Grant (P01 HL046925) NHLBI Summer Undergraduate Research Program to Increase Diversity in Research (SURP grant) (R25 HL108825) National Center for Research Resources National Institutes of Health (NIH) CTSA Grants (U54TR001356 ; UL1TR000039 ; 1S10 RR027219) Thrasher Research Fund (0285‐3)
- Language
- English
- Date published
- 08/2018
- Academic Unit
- Stead Family Department of Pediatrics; Pathology; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984366019802771
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