Journal article
Developmental Changes in Peripherin-eGFP Expression in Spiral Ganglion Neurons
Frontiers in cellular neuroscience, Vol.15, 678113
06/15/2021
DOI: 10.3389/fncel.2021.678113
Abstract
The two types of spiral ganglion neurons (SGNs), types I and II, innervate inner hair cells and outer hair cells, respectively, within the mammalian cochlea and send another process back to cochlear nuclei in the hindbrain. Studying these two neuronal types has been made easier with the identification of unique molecular markers. One of these markers, peripherin, was shown using antibodies to be present in all SGNs initially but becomes specific to type II SGNs during maturation. We used mice with fluorescently labeled peripherin (Prph-eGFP) to examine peripherin expression in SGNs during development and in aged mice. Using these mice, we confirm the initial expression of Prph-eGFP in both types I and II neurons and eventual restriction to only type II perikarya shortly after birth. However, while Prph-eGFP is uniquely expressed within type II cell bodies by P8, both types I and II peripheral and central processes continue to express Prph-eGFP for some time before becoming downregulated. Only at P30 was there selective type II Prph-eGFP expression in central but not peripheral processes. By 9 months, only the type II cell bodies and more distal central processes retain Prph-eGFP expression. Our results show that Prph-eGFP is a reliable marker for type II SGN cell bodies beyond P8; however, it is not generally a suitable marker for type II processes, except for central processes beyond P30. How the changes in Prph-eGFP expression relate to subsequent protein expression remains to be explored.
Details
- Title: Subtitle
- Developmental Changes in Peripherin-eGFP Expression in Spiral Ganglion Neurons
- Creators
- Karen L ElliottJennifer KersigoJeong Han LeeIsrat JahanGabriela PavlinkovaBernd FritzschEbenezer N Yamoah
- Resource Type
- Journal article
- Publication Details
- Frontiers in cellular neuroscience, Vol.15, 678113
- DOI
- 10.3389/fncel.2021.678113
- ISSN
- 1662-5102
- eISSN
- 1662-5102
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: R01 AG060504, P01 AG051443, R01 DC015135, R01 DC016099, R01 DC015252-01
- Language
- English
- Date published
- 06/15/2021
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984090886202771
Metrics
24 Record Views