Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices

Joseph Glykys; Kevin J Staley

doi:10.1371/journal.pone.0158012

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Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices

Journal article

Open access

Peer reviewed

Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices

Joseph Glykys and Kevin J Staley

PloS one, Vol.11(6), pp.e0158012-e0158012

2016

DOI: 10.1371/journal.pone.0158012

PMCID: PMC4919081

PMID: 27337272

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Abstract

The intraneuronal chloride concentration ([Cl-]i) is paramount for determining the polarity of signaling at GABAA synapses in the central nervous system. Sectioning hippocampal brain slices increases [Cl-]i in the superficial layers. It is not known whether cutting trauma also increases [Cl-]i in the neocortex and thalamus, and whether the effects of trauma change during development. We used Cl- imaging to study the [Cl-]i vs. the distance from the cut surface in acute thalamocortical slices from mice at developmental ages ranging from post-natal day 5 (P5) to P20. We demonstrate: 1) [Cl-]i is higher in the most superficial areas in both neocortical and thalamic brain slices at all ages tested and, 2) there is a developmental decrease in [Cl-]i that is independent of acute trauma caused by brain slicing. We conclude that [Cl-]i has a developmental progression during P5-20 in both the neocortex and thalamus. However, in both brain regions and during development the neurons closest to the slicing trauma have an elevated [Cl-]i.

Neocortex - pathology

Neocortex - metabolism

Animals

Brain Injuries, Traumatic - metabolism

Brain Injuries, Traumatic - pathology

Thalamus - metabolism

Chlorides - metabolism

Mice

Neurons - metabolism

Disease Progression

Disease Models, Animal

Details

Title: Subtitle: Developmental Decrease of Neuronal Chloride Concentration Is Independent of Trauma in Thalamocortical Brain Slices
Creators: Joseph Glykys - Harvard Medical School, Boston, Massachusetts, United States of America
Kevin J Staley - Harvard Medical School, Boston, Massachusetts, United States of America
Resource Type: Journal article
Publication Details: PloS one, Vol.11(6), pp.e0158012-e0158012
DOI: 10.1371/journal.pone.0158012
PMID: 27337272
PMCID: PMC4919081
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: R01 NS040109 / NINDS NIH HHS K08 NS091248 / NINDS NIH HHS U01 MH106013 / NIMH NIH HHS
Language: English
Date published: 2016
Academic Unit: Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurology (Pediatrics)
Record Identifier: 9984018933102771

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