Journal article
Developmental Regulation of the α-Glycerophosphate Shuttle in Porcine Myocardium
Journal of molecular and cellular cardiology, Vol.29(6), pp.1605-1613
06/1997
DOI: 10.1006/jmcc.1997.0394
PMID: 9220346
Abstract
The α-glycerophosphate (α-GP) shuttle has been shown to play a role in reducing equivalent transfer in neonatal cardiac mitochondria. In adult heart mitochondria, α-GP shuttle activity is not detectable. The goals of the current study were to define the time course of the age-dependent decline in α-GP shuttle capacity and to identify the enzymatic step(s) of the α-GP shuttle which are regulated during development. Intact mitochondria were isolated from porcine hearts of various ages and assayed for α-GP shuttle capacity. By 5 weeks of age, α-GP shuttle capacity had decreased by nearly 39%. The cytosolic step of the shuttle, catalysed by cytosolic α-glycerophosphate dehydrogenase (cα-GPDH), demonstrated a significant increase between 0–2-day-old animals and adults. Partial cDNA clones of porcine cα-GPDH and mitochondrial α-glycerophosphate dehydrogenase (mα-GPDH) were prepared and used to quantitate expression of these genes. Using mRNA isolated from neonatal and adult porcine myocardium, expression of the cα-GPDH was unchanged, while expression of the mα-GPDH gene was present in neonatal but absent in adult myocardium. These results demonstrate a rapid postnatal decline in myocardial α-GP shuttle capacity which appears to be regulated by a decline in mα-GPDH gene expression.
Details
- Title: Subtitle
- Developmental Regulation of the α-Glycerophosphate Shuttle in Porcine Myocardium
- Creators
- Thomas D ScholzStacia L KoppenhaferCynthia J TenEyckBrian C Schutte
- Resource Type
- Journal article
- Publication Details
- Journal of molecular and cellular cardiology, Vol.29(6), pp.1605-1613
- Publisher
- Elsevier Ltd
- DOI
- 10.1006/jmcc.1997.0394
- PMID
- 9220346
- ISSN
- 0022-2828
- eISSN
- 1095-8584
- Language
- English
- Date published
- 06/1997
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics; Hematology/Oncology; Fraternal Order of Eagles Diabetes Research Center; Child and Community Health
- Record Identifier
- 9984093353302771
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