Developmental changes in nuclear lamina components during germ cell differentiation

Isabella E Perales; Samuel D Jones; Katherine M Piaszynski; Pamela K Geyer

doi:10.1080/19491034.2024.2339214

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Developmental changes in nuclear lamina components during germ cell differentiation

Journal article

Open access

Peer reviewed

Developmental changes in nuclear lamina components during germ cell differentiation

Isabella E Perales, Samuel D Jones, Katherine M Piaszynski and Pamela K Geyer

Nucleus (Austin, Tex.), Vol.15(1), 2339214

04/10/2024

DOI: 10.1080/19491034.2024.2339214

PMCID: PMC11008544

PMID: 38597409

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https://doi.org/10.1080/19491034.2024.2339214View

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Abstract

The nuclear lamina (NL) changes composition for regulation of nuclear events. We investigated changes that occur in Drosophila oogenesis, revealing switches in NL composition during germ cell differentiation. Germline stem cells (GSCs) express only LamB and predominantly emerin, whereas differentiating nurse cells predominantly express LamC and emerin2. A change in LamC-specific localization also occurs, wherein phosphorylated LamC redistributes to the nuclear interior only in the oocyte, prior to transcriptional reactivation of the meiotic genome. These changes support existing concepts that LamC promotes differentiation, a premise that was tested. Remarkably ectopic LamC production in GSCs did not promote premature differentiation. Increased LamC levels in differentiating germ cells altered internal nuclear structure, increased RNA production, and reduced female fertility due to defects in eggshell formation. These studies suggest differences between Drosophila lamins are regulatory, not functional, and reveal an unexpected robustness to level changes of a major scaffolding component of the NL.

Cell Differentiation

Animals

Drosophila

Drosophila melanogaster - genetics

Drosophila Proteins - genetics

Female

Germ Cells

Nuclear Lamina

Details

Title: Subtitle: Developmental changes in nuclear lamina components during germ cell differentiation
Creators: Isabella E Perales - University of Iowa
Samuel D Jones - University of Iowa
Katherine M Piaszynski - University of Iowa
Pamela K Geyer - University of Iowa
Resource Type: Journal article
Publication Details: Nucleus (Austin, Tex.), Vol.15(1), 2339214
DOI: 10.1080/19491034.2024.2339214
PMID: 38597409
PMCID: PMC11008544
NLM abbreviation: Nucleus
ISSN: 1949-1034
eISSN: 1949-1042
Grant note: name: the National Institute of Health, award: GM087341
Language: English
Date published: 04/10/2024
Academic Unit: Neurology; Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984584906502771

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