Journal article
Developmental expression and distribution of sheep β-defensin-2
Developmental and comparative immunology, Vol.28(2), pp.171-178
2004
DOI: 10.1016/S0145-305X(03)00105-8
PMID: 12969802
Abstract
The aim of this study was to define the ontogeny of sheep β-defensin-2 (SBD-2) mRNA and peptide in selected tissues of fetal, neonatal and adult sheep by real-time PCR and immunohistochemistry, respectively. Fetal and neonatal lambs had significantly greater SBD-2 tissue distribution than adult sheep. For all ages, the intestines had consistent SBD-2 mRNA expression while extra intestinal expression was sporadic and weak. In adult sheep, SBD-2 mRNA levels decreased from the jejunum caudally to the rectum and a pooled sample from all age groups showed a similar tendency. SBD-2 immunoreactive cells were predominantly in the crypts and base of villi in the small intestine and in a modest number of glands in the large intestine. Interestingly, ileal follicle-associated epithelium lacked detectable SBD-2 immunoreactivity. SBD-2 mRNA and peptide expression are greatest in the intestinal tract and tissue distribution progressively decreases with maturity.
Details
- Title: Subtitle
- Developmental expression and distribution of sheep β-defensin-2
- Creators
- David K Meyerholz - Department of Veterinary Pathology, 2740 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USAJack M Gallup - Department of Veterinary Pathology, 2740 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USABranka M Grubor - Department of Veterinary Pathology, 2740 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USARichard B Evans - Department of Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USABrian F Tack - Department of Microbiology, University of Iowa, Iowa City, IA, USAPaul B McCray Jr - Department of Pediatrics, University of Iowa, Iowa City, IA, USAMark R Ackermann - Department of Veterinary Pathology, 2740 Veterinary Medicine, Iowa State University, Ames, IA 50011-1250, USA
- Resource Type
- Journal article
- Publication Details
- Developmental and comparative immunology, Vol.28(2), pp.171-178
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/S0145-305X(03)00105-8
- PMID
- 12969802
- ISSN
- 0145-305X
- eISSN
- 1879-0089
- Language
- English
- Date published
- 2004
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Pathology; Internal Medicine
- Record Identifier
- 9984083869302771
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