Developmental expression of Kcnq4 in vestibular neurons and neurosensory epithelia

Sonia M.S Rocha-Sanchez; Kenneth A Morris; Bechara Kachar; David Nichols; Bernd Fritzsch; Kirk W Beisel

doi:10.1016/j.brainres.2006.12.087

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Developmental expression of Kcnq4 in vestibular neurons and neurosensory epithelia

Journal article

Peer reviewed

Developmental expression of Kcnq4 in vestibular neurons and neurosensory epithelia

Sonia M.S Rocha-Sanchez, Kenneth A Morris, Bechara Kachar, David Nichols, Bernd Fritzsch and Kirk W Beisel

Brain research, Vol.1139(1), pp.117-125

2007

DOI: 10.1016/j.brainres.2006.12.087

PMCID: PMC1858668

PMID: 17292869

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http://doi.org/10.1016/j.brainres.2006.12.087View

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Abstract

Sensory signal transduction of the inner ear afferent neurons and hair cells (HCs) requires numerous ionic conductances. The KCNQ4 voltage-gated M-type potassium channel is thought to set the resting membrane potential in cochlear HCs. Here we describe the spatiotemporal expression patterns of\nKcnq4 and the associated alternative splice forms in the HCs of vestibular labyrinth. Whole mount immunodetection, qualitative and quantitative RT-PCR were performed to characterize the expression patterns of\nKcnq4 transcripts and proteins. A topographical expression and upregulation of\nKcnq4 during development was observed and indicated that\nKcnq4 is not restricted to either a specific vestibular structure or cell type, but is present in afferent calyxes, vestibular ganglion neurons, and both type I and type II HCs. Of the four alternative splice variants,\nKcnq4_v1 transcripts were the predominant form in the HCs, while\nKcnq4_v3 was the major variant in the vestibular neurons. Differential quantitative expression of\nKcnq4_v1 and\nKcnq4_v3 were respectively detected in the striolar and extra-striolar regions of the utricle and saccule. Analysis of gerbils and rats yielded results similar to those obtained in mice, suggesting that the spatiotemporal expression pattern of\nKcnq4 in the vestibular system is conserved among rodents. Analyses of vestibular HCs of\nBdnf conditional mutant mice, which are devoid of any innervation, demonstrate that regulation of\nKcnq4 expression in vestibular HCs is independent of innervation.

Immunohistochemistry

Kcnq4

RT-PCR

Vestibular hair cell

Ion channel

Immunofluorescence

In situ hybridization

Alternative splice variant

Details

Title: Subtitle: Developmental expression of Kcnq4 in vestibular neurons and neurosensory epithelia
Creators: Sonia M.S Rocha-Sanchez - Department of Oral Biology, Creighton University School of Dentistry, 2500 California Plaza, Omaha, NE 68178, USA
Kenneth A Morris - Department of Biomedical Sciences, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA
Bechara Kachar - Structural Cell Biology Section, Laboratory of Cell Biology, NICD/NIH, Bethesda, MD 20892, USA
David Nichols - Department of Biomedical Sciences, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA
Bernd Fritzsch - Department of Biomedical Sciences, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA
Kirk W Beisel - Department of Biomedical Sciences, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA
Resource Type: Journal article
Publication Details: Brain research, Vol.1139(1), pp.117-125
DOI: 10.1016/j.brainres.2006.12.087
PMID: 17292869
PMCID: PMC1858668
NLM abbreviation: Brain Res
ISSN: 0006-8993
eISSN: 1872-6240
Publisher: Elsevier B.V
Language: English
Date published: 2007
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070154402771

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