Journal article
Developmental expression of catenins and associated proteins during submucosal gland morphogenesis in the airway
Experimental lung research, Vol.27(2), pp.121-141
2001
DOI: 10.1080/019021401750069375
PMID: 11258801
Abstract
Although lymphoid enhancer binding factor-1 (Lef-1) plays an obligatory role in airway submucosal gland (SMG) development, its expression alone is not an adequate signal for initiating gland morphogenesis. Because Lef-1 forms a bipartite transcription factor with beta-catenin to mediate wnt pathway signaling, we investigated the expression of beta-catenin and associated proteins during SMG development with both in situ hybridization and immunocytochemistry. Unexpectedly, high levels of E-cadherin mRNA were expressed by cells in developing gland buds from the earliest stages through subsequent differentiation into mature glands. In contrast, a decreased level of E-cadherin immunoreactivity in stage I gland bud cells suggested that post-translational modulation of E-cadherin protein levels may play a critical role in early stages of gland morphogenesis. Adenomatous polyposis coli (APC) mRNA was expressed relatively weakly in the developing ferret trachea, but higher levels of protein staining were observed throughout the cytoplasm of gland buds and surface epithelial cells. B-Catenin mRNA was abundantly expressed throughout the tracheal epithelium and at the highest levels in primordial gland buds. B-Catenin protein localized to the basolateral membranes of all airway epithelial cell types. However, no detectable increases in nuclear or cytoplasmic staining were associated with gland buds, as would be expected if beta-catenin served as a transcriptional cofactor for Lef-1 in gland morphogenesis. Additional studies demonstrated the gamma-catenin distribution to be remarkably similar to that of beta-catenin, whereas alpha-catenin staining was more diffuse in the cytoplasm of airway epithelial and gland bud cells. These descriptive results do not rule out a role for wnt signaling in SMG development , but provide no evidence that beta-catenin, or gamma-catenin, is a cofactor in Lef-1 regulation of SMG development.
Details
- Title: Subtitle
- Developmental expression of catenins and associated proteins during submucosal gland morphogenesis in the airway
- Creators
- Teresa C RITCHIE - Department of Anatomy and Cell Biology, The University of Iowa, School of Medicine, Iowa City, Iowa, United StatesWeihong Zhou - Department of Anatomy and Cell Biology, The University of Iowa, School of Medicine, Iowa City, Iowa, United StatesElizabeth MCKINSTRY - Department of Anatomy and Cell Biology, The University of Iowa, School of Medicine, Iowa City, Iowa, United StatesMicaela HOSCH - Department of Anatomy and Cell Biology, The University of Iowa, School of Medicine, Iowa City, Iowa, United StatesYulong Zhang - Department of Anatomy and Cell Biology, The University of Iowa, School of Medicine, Iowa City, Iowa, United StatesInke NATHKE - Department of Anatomy and Physiology, The University of Dundee, Dundee, Scotland, United KingdomJohn F ENGELHARDT - Department of Anatomy and Cell Biology and Department of Internal Medicine, The University of Iowa, School of Medicine, Iowa City, Iowa, United States
- Resource Type
- Journal article
- Publication Details
- Experimental lung research, Vol.27(2), pp.121-141
- Publisher
- Taylor & Francis; Philadelphia, PA
- DOI
- 10.1080/019021401750069375
- PMID
- 11258801
- ISSN
- 0190-2148
- eISSN
- 1521-0499
- Language
- English
- Date published
- 2001
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025592202771
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