Journal article
Developmental expression of the epithelial Na+ channel in kidney and uroepithelia
American journal of physiology. Renal physiology, Vol.276(2), pp.F304-F314
02/01/1999
DOI: 10.1152/ajprenal.1999.276.2.F304
PMID: 9950962
Abstract
The epithelial Na+ channel (ENaC) plays an important role in regulating Na+ balance in neonatal and adult life. Using in situ hybridization, we localized α-, β-, and γ-rat ENaC (rENaC) mRNA in developing rat kidney and uroepithelia. rENaC mRNA was first detectable on fetal day 16, and by fetal day 17, mRNA was abundant in the terminal collecting duct and uroepithelia. After birth, the intensity of the signals for all three subunits increased in the cortical collecting ducts and by 9 days after birth had diminished in the inner medullary collecting ducts. Expression in uroepithelial cells was different. mRNA for β- and γ-rENaC, but not α-rENaC, was detected in pelvis, ureters, and bladder at all stages of development beyond fetal day 16. By RNase protection assay (RPA), the greatest increase in subunit abundance in the kidney occurred before birth. Between postnatal days 9 and 30, the abundance of β- and γ-rENaC decreased relative to α-rENaC in outer and inner medulla. The urinary bladder, in contrast, demonstrated the greatest increase in β- and γ-rENaC mRNA abundance after birth. We were generally unable to detect α-rENaC by RPA in urinary bladder. Feeding weaned rats a diet of high or low NaCl did not change the abundance of any of the subunit mRNAs in bladder. These results demonstrate additional heterogeneity of developmental expression and regulation of ENaC. The differences between the collecting duct and uroepithelial cell rENaC mRNA regulation raise the possibility of significant differences in function.
Details
- Title: Subtitle
- Developmental expression of the epithelial Na+ channel in kidney and uroepithelia
- Creators
- Shigeru Watanabe - Laboratory of Epithelial Transport, Departments of Internal Medicine and Pediatrics, University of Iowa; and Veterans Affairs Medical Center, Iowa City, Iowa 52242Kazumichi Matsushita - Laboratory of Epithelial Transport, Departments of Internal Medicine and Pediatrics, University of Iowa; and Veterans Affairs Medical Center, Iowa City, Iowa 52242Paul B McCray - Laboratory of Epithelial Transport, Departments of Internal Medicine and Pediatrics, University of Iowa; and Veterans Affairs Medical Center, Iowa City, Iowa 52242John B Stokes - Laboratory of Epithelial Transport, Departments of Internal Medicine and Pediatrics, University of Iowa; and Veterans Affairs Medical Center, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Renal physiology, Vol.276(2), pp.F304-F314
- DOI
- 10.1152/ajprenal.1999.276.2.F304
- PMID
- 9950962
- NLM abbreviation
- Am J Physiol Renal Physiol
- ISSN
- 1931-857X
- eISSN
- 1522-1466
- Language
- English
- Date published
- 02/01/1999
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984093460702771
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