Developmental regulation of angiotensinogen gene expression in sheep

Ann Louise Olson; Stanley Perlman; Jean E Robillard

doi:10.1203/00006450-199009000-00001

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Developmental regulation of angiotensinogen gene expression in sheep

Journal article

Open access

Peer reviewed

Developmental regulation of angiotensinogen gene expression in sheep

Ann Louise Olson, Stanley Perlman and Jean E Robillard

Pediatric research, Vol.28(3), pp.183-185

09/1990

DOI: 10.1203/00006450-199009000-00001

PMID: 2235111

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https://doi.org/10.1203/00006450-199009000-00001View

Published (Version of record) Open Access

Abstract

It has been suggested that the liver is not the main source of angiotensinogen during fetal life in rats, but that the kidney is an important site of fetal angiotensinogen synthesis. In an effort to determine if this phenomenon is specific to the rat or applicable to other species, we compared the ontogenic changes in hepatic and renal angiotensinogen mRNA expression in fetal (60, 90, 118, and 138 d of gestation, term being 145 d), newborn (7 d postnatal), and adult sheep. Total RNA was extracted, subjected to Northern blotting and hybridized using a full-length rat radiolabeled antisense RNA. Angiotensinogen mRNA sequences were detected in all fetal liver samples and appeared to increase 3-fold from 60 to 138 d gestation and then to decrease after birth. In contrast, angiotensinogen mRNA could not be detected in renal cortical tissue of 118 or 138 d fetuses, or newborn or adult sheep. We conclude that, unlike in the rat, liver angiotensinogen gene expression is detectable during the 2nd trimester of gestation in sheep and is developmentally regulated. Furthermore, in contrast to the fetal rat, angiotensinogen mRNA sequences were undetectable in fetal sheep kidney.

Pregnancy

Animals, Newborn

Liver - metabolism

RNA, Messenger - genetics

Fetus - metabolism

Gene Expression Regulation

RNA, Messenger - metabolism

Sheep - growth & development

Angiotensinogen - genetics

Kidney - metabolism

Animals

Female

Sheep - genetics

Details

Title: Subtitle: Developmental regulation of angiotensinogen gene expression in sheep
Creators: Ann Louise Olson - Department of Pediatrics, University of Iowa, College of Medicine, Iowa City 52242
Stanley Perlman
Jean E Robillard
Resource Type: Journal article
Publication Details: Pediatric research, Vol.28(3), pp.183-185
DOI: 10.1203/00006450-199009000-00001
PMID: 2235111
NLM abbreviation: Pediatr Res
ISSN: 0031-3998
eISSN: 1530-0447
Publisher: United States
Grant note: HD-20576 / NICHD NIH HHS HL-35600 / NHLBI NIH HHS DK-38302 / NIDDK NIH HHS
Language: English
Date published: 09/1990
Academic Unit: Molecular Physiology and Biophysics; Microbiology and Immunology; Nephrology, Dialysis and Transplantation; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medicine Administration; Infectious Disease (Pediatrics)
Record Identifier: 9983777348802771

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