Developmentally regulated expression of a novel 59-kDa product of the major surface protease (Msp or gp63) gene family of Leishmania chagasi

Sigrid C Roberts; Mary E Wilson; John E Donelson

doi:10.1074/jbc.270.15.8884

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Developmentally regulated expression of a novel 59-kDa product of the major surface protease (Msp or gp63) gene family of Leishmania chagasi

Journal article

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Peer reviewed

Developmentally regulated expression of a novel 59-kDa product of the major surface protease (Msp or gp63) gene family of Leishmania chagasi

Sigrid C Roberts, Mary E Wilson and John E Donelson

The Journal of biological chemistry, Vol.270(15), pp.8884-8892

04/14/1995

DOI: 10.1074/jbc.270.15.8884

PMID: 7721796

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Abstract

All species of Leishmania express a major surface protease (Msp or gp63) that facilitates the interactions of the parasite with its environment at several steps in its life cycle. The msp gene family in Leishmania chagasi contains three classes of genes whose mRNAs are differentially expressed during parasite growth. Logarithmic phase (low infectivity) promastigotes express only 63-kDa versions of Msp, whereas stationary phase (high infectivity) promastigotes express both 63- and 59-kDa Msps. The different migrations of the 59- and 63-kDa proteins on acrylamide gels are not due to differences in N-linked glycosylation or the membrane anchor. Plasmid transfections of Leishmania demonstrate that mspS2 of the stationary gene class encodes a 59-kDa protein. Expression of the 59-kDa protein in stationary phase promastigotes ceases after about 12 weeks of in vitro cultivation when the parasites become attenuated. Attenuated parasites can be stimulated to re-express the 59-kDa Msp by passage through mice followed by several in vitro passages of recovered promastigotes. Amastigotes express yet another subset of Msp proteins. Thus, the 59-kDa product of mspS2 is expressed only in stationary phase promastigotes and only after recent exposure to environmental changes encountered in the mammalian host cell.

Metalloendopeptidases - genetics

Amino Acid Sequence

Antibody Specificity

Multigene Family

Antibodies, Protozoan - immunology

Molecular Sequence Data

Metalloendopeptidases - metabolism

Glycosylation

Metalloendopeptidases - immunology

Leishmania infantum - enzymology

Hydrolysis

Animals

Glycosylphosphatidylinositols - metabolism

Gene Expression Regulation, Developmental

Leishmania infantum - genetics

Details

Title: Subtitle: Developmentally regulated expression of a novel 59-kDa product of the major surface protease (Msp or gp63) gene family of Leishmania chagasi
Creators: Sigrid C Roberts - Department of Biochemistry, University of Iowa, Iowa City, USA
Mary E Wilson
John E Donelson
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.270(15), pp.8884-8892
DOI: 10.1074/jbc.270.15.8884
PMID: 7721796
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: AI30126 / NIAID NIH HHS AI32135 / NIAID NIH HHS
Language: English
Date published: 04/14/1995
Academic Unit: Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984001113702771

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