Journal article
Dexamethasone inhibits IL-1 and TNF activity in human lung fibroblasts without affecting IL-1 or TNF receptors
American journal of physiology. Lung cellular and molecular physiology, Vol.267(1), pp.L33-L38
07/01/1994
DOI: 10.1152/ajplung.1994.267.1.L33
PMID: 7519403
Abstract
Interleukin (IL-1) and tumor necrosis factor (TNF) activate human lung fibroblasts through interactions with specific receptors. One effect of this interaction of IL-1 and TNF with fibroblasts is an increased production of the cytokines, IL-6 and IL-8. Dexamethasone blocks the induction of IL-6 and IL-8 by IL-1 or TNF. In these studies, we determined whether dexamethasone interferes with the upregulation of IL-6 and IL-8 by downregulating expression of the IL-1 or TNF receptor genes. Confluent lung fibroblasts were treated with medium alone (control) or medium with dexamethasone (10(-6) M). Dexamethasone did not decrease the binding of IL-1 and TNF to their receptors, nor did it decrease amounts of IL-1 or TNF receptor RNA. Both IL-1 and TNF increased release of IL-6 and IL-8 from the cells in a dose-dependent manner and dexamethasone inhibited this effect. Dexamethasone also inhibited the induction of IL-6 and IL-8 RNA by IL-1 and TNF. The studies show that dexamethasone does not block the effects of IL-1 or TNF on fibroblasts by decreasing expression of IL-1 or TNF receptors.
Details
- Title: Subtitle
- Dexamethasone inhibits IL-1 and TNF activity in human lung fibroblasts without affecting IL-1 or TNF receptors
- Creators
- Martha M Monick - Department of Medicine, University of Iowa College of Medicine, IowaCity 52242Timothy R Aksamit - Department of Medicine, University of Iowa College of Medicine, IowaCity 52242Lois J Geist - Department of Medicine, University of Iowa College of Medicine, IowaCity 52242Gary W Hunninghake - Department of Medicine, University of Iowa College of Medicine, IowaCity 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.267(1), pp.L33-L38
- DOI
- 10.1152/ajplung.1994.267.1.L33
- PMID
- 7519403
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Language
- English
- Date published
- 07/01/1994
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Provost Office Administration; Internal Medicine
- Record Identifier
- 9984213294002771
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