Journal article
Diagnosis of pregnancy disorder in the first‐trimester patient plasma with Raman spectroscopy and protein analysis
Bioengineering & translational medicine, Vol.9(6), e10691
11/2024
DOI: 10.1002/btm2.10691
PMCID: PMC11558203
PMID: 39545096
Abstract
Abstract Gestational diabetes mellitus (GDM) is a pregnancy disorder associated with short‐ and long‐term adverse outcomes in both mothers and infants. The current clinical test of blood glucose levels late in the second trimester is inadequate for early detection of GDM. Here we show the utility of Raman spectroscopy (RS) for rapid and highly sensitive maternal metabolome screening for GDM in the first trimester. Key metabolites, including phospholipids, carbohydrates, and major amino acids, were identified with RS and validated with mass spectrometry, enabling insights into associated metabolic pathway enrichment. Using classical machine learning (ML) approaches, we showed the performance of the RS metabolic model (cross‐validation AUC 0.97) surpassed that achieved with patients' clinical data alone (cross‐validation AUC 0.59) or prior studies with single biomarkers. Further, we analyzed novel proteins and identified fetuin‐A as a promising candidate for early GDM prediction. A correlation analysis showed a moderate to strong correlation between multiple metabolites and proteins, suggesting a combined protein‐metabolic analysis integrated with ML would enable a powerful screening platform for first trimester diagnosis. Our study underscores RS metabolic profiling as a cost‐effective tool that can be integrated into the current clinical workflow for accurate risk stratification of GDM and to improve both maternal and neonatal outcomes.
Details
- Title: Subtitle
- Diagnosis of pregnancy disorder in the first‐trimester patient plasma with Raman spectroscopy and protein analysis
- Creators
- Ansuja P. Mathew - Iowa State UniversityGabriel Cutshaw - Iowa State UniversityOlivia Appel - Iowa State UniversityMeghan Funk - University of IowaLilly Synan - Iowa State UniversityJoshua Waite - Iowa State UniversitySaman Ghazvini - Iowa State UniversityXiaona Wen - Iowa State UniversitySoumik Sarkar - Iowa State UniversityMark Santillan - University of IowaDonna Santillan - University of IowaRizia Bardhan - Iowa State University
- Resource Type
- Journal article
- Publication Details
- Bioengineering & translational medicine, Vol.9(6), e10691
- DOI
- 10.1002/btm2.10691
- PMID
- 39545096
- PMCID
- PMC11558203
- NLM abbreviation
- Bioeng Transl Med
- ISSN
- 2380-6761
- eISSN
- 2380-6761
- Language
- English
- Electronic publication date
- 07/16/2024
- Date published
- 11/2024
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9984658256702771
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