Journal article
Diagnostic Utility of Isolated Tube C Positivity in T-Cell Receptor Testing Using BIOMED-2 Primers
American journal of clinical pathology, Vol.151(4), pp.386-394
04/01/2019
DOI: 10.1093/ajcp/aqy157
PMID: 30534953
Abstract
Objectives T-cell receptor (TCR) gene rearrangement studies are widely used for assessing T-cell clonality. The frequency and significance of clonal peaks restricted to TCR (TCRB) tube C are uncertain. We retrospectively reviewed 80 TCR studies performed on bone marrow/peripheral blood.
Methods TCRB and TCR (TCRG) analyses were performed using BIOMED-2 primers. A peak was considered clonal or atypical if it was reproducible and 5x or more or 3x to 5x polyclonal background, respectively.
Results TCRB analysis demonstrated 12 (15%) of 80 cases with one to four isolated peaks in tube C (>3x) with polyclonal pattern in tubes A and B. TCRG analysis was monoclonal in two cases (both definite T-cell neoplasms), polyclonal in four, and oligoclonal in six. Of the 10 cases without clone in TCRG, six had autoimmune disorder and none had T-cell neoplasm.
Conclusions Peaks restricted to TCRB tube C in the TCR analysis may be misleading, as it is often not indicative of an overt T-cell neoplasm.
Details
- Title: Subtitle
- Diagnostic Utility of Isolated Tube C Positivity in T-Cell Receptor Testing Using BIOMED-2 Primers
- Creators
- Sohail Qayyum - University of PittsburghGrant C. Bullock - University of PittsburghSteven H. Swerdlow - University of PittsburghRaven Brower - University of PittsburghMarina Nikiforova - University of PittsburghNidhi Aggarwal - University of Pittsburgh
- Resource Type
- Journal article
- Publication Details
- American journal of clinical pathology, Vol.151(4), pp.386-394
- Publisher
- Oxford Univ Press
- DOI
- 10.1093/ajcp/aqy157
- PMID
- 30534953
- ISSN
- 0002-9173
- eISSN
- 1943-7722
- Number of pages
- 9
- Language
- English
- Date published
- 04/01/2019
- Academic Unit
- Pathology
- Record Identifier
- 9984697739102771
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