Journal article
Diarrheagenic Escherichia coli and Acute Gastroenteritis in Children in Davidson County, Tennessee, United States: A Case-control Study
The Pediatric infectious disease journal, Vol.37(6), pp.543-548
06/2018
DOI: 10.1097/INF.0000000000001908
PMCID: PMC5962020
PMID: 29341983
Abstract
Diarrheagenic Escherichia coli (DEC) is an important cause of acute gastroenteritis in children; however, there is limited information available on the epidemiology, phylogenetics, serotyping and antibiotic susceptibility of DEC in children in the United States. The aim of this study was to determine the molecular epidemiology of DEC among children with and without acute gastroenteritis in Davidson County, Tennessee.
This prospective, frequency matched, case-control study recruited subjects 15 days to 17 years of age and detected DEC with polymerase chain reaction from stool samples. Additional testing was done to define phylogenetics and antibiotics resistance.
Among 1267 participants, 857 cases and 410 controls, 5.5% were positive for at least one subtype of DEC. Enteroaggregative E. coli [n = 32 (45%)] was the most common subtype followed by enteropathogenic E. coli (EPEC) [n = 30 (43%)], Shiga toxin-producing E. coli [n = 4 (6%)] and diffusely adherent E. coli [n = 4 (6%)]. No significant difference in prevalence of DEC was found between cases (5%) and controls (7%) [odds ratio: 0.66 (95% confidence interval: 0.4-1.07)], and results were similar when data were stratified by subtypes and adjusted for age, sex, race and ethnicity. Substantial diversity was found among DEC isolates in terms of phylotypes and serotypes, and a large proportion was resistant to, at least, one antibiotic.
Enteroaggregative E. coli and enteropathogenic E. coli were frequently found in both cases and controls in this study population. DNA-based methods for detection of these subtypes need further investigation to help differentiate between pathogenic and colonizing strains.
Details
- Title: Subtitle
- Diarrheagenic Escherichia coli and Acute Gastroenteritis in Children in Davidson County, Tennessee, United States: A Case-control Study
- Creators
- Aamer Imdad - From the D. Brent Polk Division of Pediatric GastroenterologyMonique A Foster - Pediatric Infectious Diseases SocietyJunaid Iqbal - Pediatric Infectious Diseases SocietyChristopher Fonnesbeck - Vanderbilt UniversityDaniel C Payne - Centers for Disease Control and PreventionChengxian Zhang - Pediatric Infectious Diseases SocietyJames D Chappell - Pediatric Infectious Diseases SocietyNatasha Halasa - Pediatric Infectious Diseases SocietyOscar G Gómez-Duarte - Pediatric Infectious Diseases Society
- Resource Type
- Journal article
- Publication Details
- The Pediatric infectious disease journal, Vol.37(6), pp.543-548
- DOI
- 10.1097/INF.0000000000001908
- PMID
- 29341983
- PMCID
- PMC5962020
- ISSN
- 0891-3668
- eISSN
- 1532-0987
- Grant note
- R01 AI095346 / NIAID NIH HHS U01 IP000464 / NCIRD CDC HHS UL1 TR000445 / NCATS NIH HHS U01 IP001063 / NCIRD CDC HHS
- Language
- English
- Date published
- 06/2018
- Academic Unit
- Stead Family Department of Pediatrics; Gastroenterology, Hepatology, Pancreatology, and Nutrition
- Record Identifier
- 9984446525302771
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