Journal article
Dicalcium phosphate (CaHPO4 center dot 2H(2)O) precipitation through ortho- or meta-phosphoric acid-etching: Effects on the durability and nanoleakage/ultra-morphology of resin-dentine interfaces
Journal of dentistry, Vol.41(11), pp.1068-1080
11/01/2013
DOI: 10.1016/j.jdent.2013.08.014
PMID: 23994709
Abstract
Objectives: To compare the effects of two etching procedures using meta-phosphoric (MPA) or ortho-phosphoric acid (OPA) on dentine demineralisation, resin-dentine bonds durability and interface nanoleakage/ultra-morphology.
Methods: Middle-dentine specimens were etched using 37% OPA (15 s) or 40% MPA (60 s) and submitted to infrared spectroscopy (FTIR) or ultra-morphology dye-assisted (calcium-staining) confocal microscopy (Ca-CLSM). A three-step etch-and-rinse adhesive was formulated, applied onto dentine and light-cured for 30 s before composite build-up. After 24 h, the dentine-bonded specimens were cut into 1 mm(2) beams; half were immediately submitted to microtensile bond strength (mu TBS) and half stored in DW for six months. The mu TBS results were analysed with repeated-measures ANOVA and Tukey's test (p < 0.05). Further teeth were bonded and prepared for interface nanoleakage/ultra-morphology confocal evaluation.
Results: FTIR and Ca-CLSM analyses showed dicalcium phosphate dihydrate (Brushite) precipitation in MPA-etched dentine and on the bottom (front of demineralisation) of the OPA-etched dentine. Statistical analysis showed similar mu TBS for both etching procedures after 24 h. The mu TBS of specimens in OPA-group dropped significantly (p < 0.05) after six month; the specimens in the MPA group showed no statistically difference (p > 0.05). CLSM depicted no evident sign of nanoleakage within the resin-dentine interface of the MPA-treated specimens, while the specimens in OPA-group presented intense nanoleakage and interface degradation.
Conclusion: The use of MPA (60 s) as an alternative dentine conditioning agent in etch-and-rinse bonding procedures may be a suitable strategy to create more durable resin-dentine bonds. (C) 2013 Elsevier Ltd. All rights reserved.
Details
- Title: Subtitle
- Dicalcium phosphate (CaHPO4 center dot 2H(2)O) precipitation through ortho- or meta-phosphoric acid-etching: Effects on the durability and nanoleakage/ultra-morphology of resin-dentine interfaces
- Creators
- Victor Pinheiro Feitosa - Piracicaba Dent Sch, Div Dent Mat, Piracicaba, BrazilMaria Giulia Bazzocchi - University of BolognaAngelo Putignano - Polytech Univ Marche, Dept Clin Sci & Stomatol, Ancona, ItalyGiovanna Orsini - Polytech Univ Marche, Dept Clin Sci & Stomatol, Ancona, ItalyArlinda Luzi Luzi - Univ CEU Cardenal Herrera Valencia, Dept Restorat Dent & Endodont, Valencia 46115, SpainMario Alexandre Coelho Sinhoreti - Piracicaba Dent Sch, Div Dent Mat, Piracicaba, BrazilTimothy F. Watson - Kings Coll London, Inst Dent, London, EnglandSalvatore Sauro - Kings Coll London, Inst Dent, London, England
- Resource Type
- Journal article
- Publication Details
- Journal of dentistry, Vol.41(11), pp.1068-1080
- Publisher
- Elsevier
- DOI
- 10.1016/j.jdent.2013.08.014
- PMID
- 23994709
- ISSN
- 0300-5712
- eISSN
- 1879-176X
- Number of pages
- 13
- Grant note
- NE/K012207/1 / Natural Environment Research Council; UK Research & Innovation (UKRI); Natural Environment Research Council (NERC) Wellcome Trust; European Commission Centre of Excellence in Medical Engineering II-3A-1109-10046 / National Institute for Health Research; National Institute for Health Research (NIHR) NE/K012207/1 / NERC; UK Research & Innovation (UKRI); Natural Environment Research Council (NERC) 6850-12-0 / Ministry of Education of Brazil (Capes); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Language
- English
- Date published
- 11/01/2013
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984654471102771
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