Journal article
Diet-Induced Hyperinsulinemia Accelerates Growth of Androgen-Independent PC-3 Cells In Vitro
Nutrition and cancer, Vol.64(1), pp.121-127
01/01/2012
DOI: 10.1080/01635581.2012.630556
PMID: 22171530
Abstract
We investigated the effect of insulin and diet-induced hyperinsulinemia on the growth of the PC-3 cells in vitro and discerned the growth promoting effect of insulin in the androgen-independent cells. Sprague-Dawley rats were kept on a high-fat diet for 4 wk for the induction of insulin resistance and hyperinsulinemia. Insulin alone or serum of the rats kept on either normal-pellet diet or high-fat diet was used to stimulate the serum-starved PC-3 cells growth in culture. S961, a high-affinity insulin-receptor antagonist, was used to confirm the insulin-mediated effects. Significant impairment in the glucose disposal rate and increase in the serum glucose and insulin levels was observed in the high-fat-diet-fed rats. The media supplemented with the serum of the high-fat-diet-fed rats accelerated the growth of the PC-3 cells in comparison to that of normal-pellet-diet-fed rats. Insulin treatment led to accelerated growth of the serum-starved PC-3 cells in a dose-dependent manner and inhibited by the S961 pretreatment. Insulin and serum of the diet-induced hyperinsulinemic rats promote the growth of androgen-independent prostate cancer PC-3 cells. Further, our results provide support for the concept that diet-associated elevation in insulin level may augment the growth of prostate cancer cells.
Details
- Title: Subtitle
- Diet-Induced Hyperinsulinemia Accelerates Growth of Androgen-Independent PC-3 Cells In Vitro
- Creators
- Ajit Vikram - National Institute of Pharmaceutical Education and ResearchGopabandhu Jena - Natl Inst Pharmaceut Educ & Res, Dept Pharmacol & Toxicol, Facil Risk Assessment & Intervent Studies, Mohali 160062, Punjab, India
- Resource Type
- Journal article
- Publication Details
- Nutrition and cancer, Vol.64(1), pp.121-127
- Publisher
- Taylor & Francis
- DOI
- 10.1080/01635581.2012.630556
- PMID
- 22171530
- ISSN
- 0163-5581
- eISSN
- 1532-7914
- Number of pages
- 7
- Grant note
- National Institute of Pharmaceutical Education and Research, S.A.S. Nagar, India
- Language
- English
- Date published
- 01/01/2012
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984359908202771
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