Journal article
Diet-induced obesity causes insulin resistance in mouse brown adipose tissue
Obesity (Silver Spring, Md.), Vol.23(9), pp.1765-1770
09/2015
DOI: 10.1002/oby.21134
PMCID: PMC4551605
PMID: 26242777
Abstract
Diet-induced obesity (DIO) causes several pathophysiological changes in adipose tissue. Increased inflammation reduces white adipose tissue (WAT) insulin sensitivity and contributes to the development of diabetes. However, little is known about how DIO alters the function of brown adipose tissue (BAT), an organ that consumes calories by β3-adrenergic receptor (AR)-mediated thermogenesis and helps regulate energy balance.
To test the effects of DIO on BAT, we fed 6-week-old C57BL/6 mice either a normal chow diet (NCD) or a high-fat diet (HFD). After 16 additional weeks, we measured body fat, WAT, and BAT mRNA expression, glucose tolerance, and rates of glucose uptake in response to insulin and the β3-AR agonist mirabegron.
Compared with NCD, HFD increased body fat and impaired glucose tolerance. Both WAT and BAT had higher mRNA levels of markers of inflammation, including TNFα and F4/80. Insulin signaling in BAT and WAT was reduced, with decreased Akt phosphorylation. Diet-normalized BAT glucose uptake rates were lower in response to mirabegron.
These results support a model in which DIO leads to BAT inflammation and insulin resistance, leading to a broader impairment of BAT function.
Details
- Title: Subtitle
- Diet-induced obesity causes insulin resistance in mouse brown adipose tissue
- Creators
- Carla Roberts-Toler - Section of Integrative Physiology and Metabolism, Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts, USABrian T O'Neill - Section of Integrative Physiology and Metabolism, Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts, USAAaron M Cypess - Translational Physiology Section, Diabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, Maryland, USA
- Resource Type
- Journal article
- Publication Details
- Obesity (Silver Spring, Md.), Vol.23(9), pp.1765-1770
- DOI
- 10.1002/oby.21134
- PMID
- 26242777
- PMCID
- PMC4551605
- ISSN
- 1930-7381
- eISSN
- 1930-739X
- Grant note
- Z99 DK999999 / Intramural NIH HHS K23 DK081604 / NIDDK NIH HHS UL1 RR025758 / NCRR NIH HHS UL1RR025758 / NCRR NIH HHS K08 DK100543 / NIDDK NIH HHS
- Language
- English
- Date published
- 09/2015
- Academic Unit
- Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094551302771
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