Journal article
Diet-induced obesity does not impact the generation and maintenance of primary memory CD8 T cells
The Journal of immunology (1950), Vol.193(12), pp.5873-5882
12/15/2014
DOI: 10.4049/jimmunol.1401685
PMCID: PMC4372810
PMID: 25378592
Abstract
The extent to which obesity compromises the differentiation and maintenance of protective memory CD8 T cell responses and renders obese individuals susceptible to infection remains unknown. In this study, we show that diet-induced obesity did not impact the maintenance of pre-existing memory CD8 T cells, including acquisition of a long-term memory phenotype (i.e., CD27(hi), CD62L(hi), KLRG1(lo)) and function (i.e., cytokine production, secondary expansion, and memory CD8 T cell-mediated protection). Additionally, obesity did not influence the differentiation and maintenance of newly evoked memory CD8 T cell responses in inbred and outbred hosts generated in response to different types of systemic (LCMV, L. monocytogenes) and/or localized (influenza virus) infections. Interestingly, the rate of naive-to-memory CD8 T cell differentiation after a peptide-coated dendritic cell immunization was similar in lean and obese hosts, suggesting that obesity-associated inflammation, unlike pathogen- or adjuvant-induced inflammation, did not influence the development of endogenous memory CD8 T cell responses. Therefore, our studies reveal that the obese environment does not influence the development or maintenance of memory CD8 T cell responses that are either primed before or after obesity is established, a surprising notion with important implications for future studies aiming to elucidate the role obesity plays in host susceptibility to infections.
Details
- Title: Subtitle
- Diet-induced obesity does not impact the generation and maintenance of primary memory CD8 T cells
- Creators
- Shaniya H Khan - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242Emily A Hemann - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242Kevin L Legge - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242; Department of Pathology, University of Iowa, Iowa City, IA 52242; andLyse A Norian - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242; Department of Urology, University of Iowa, Iowa City, IA 52242Vladimir P Badovinac - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA 52242; Department of Pathology, University of Iowa, Iowa City, IA 52242; and vladimir-badovinac@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.193(12), pp.5873-5882
- DOI
- 10.4049/jimmunol.1401685
- PMID
- 25378592
- PMCID
- PMC4372810
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- United States
- Grant note
- AI83286 / NIAID NIH HHS R01 AI083286 / NIAID NIH HHS T32 AI007485 / NIAID NIH HHS R01 CA181088 / NCI NIH HHS P30CA086862 / NCI NIH HHS R21 AI096850 / NIAID NIH HHS P30 CA086862 / NCI NIH HHS
- Language
- English
- Date published
- 12/15/2014
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047675102771
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