Journal article
Dietary fat, fatty acid saturation and mitochondrial bioenergetics
Journal of bioenergetics and biomembranes, Vol.46(1), pp.33-44
02/2014
DOI: 10.1007/s10863-013-9530-z
PMCID: PMC3955408
PMID: 24121995
Abstract
Fat intake alters mitochondrial lipid composition which can affect function. We used novel methodology to assess bioenergetics, including simultaneous ATP and reactive oxygen species (ROS) production, in liver and heart mitochondria of C57BL/6 mice fed diets of variant fatty acid content and saturation. Our methodology allowed us to clamp ADP concentration and membrane potential (ΔΨ) at fixed levels. Mice received a control diet for 17–19 weeks, a high-fat (HF) diet (60% lard) for 17–19 weeks, or HF for 12 weeks followed by 6–7 weeks of HF with 50% of fat as menhaden oil (MO) which is rich in
n-3
fatty acids. ATP production was determined as conversion of 2-deoxyglucose to 2-deoxyglucose phosphate by NMR spectroscopy. Respiration and ATP production were significantly reduced at all levels of ADP and resultant clamped ΔΨ in liver mitochondria from mice fed HF compared to controls. At given ΔΨ, ROS production per mg mitochondrial protein, per unit respiration, or per ATP generated were greater for liver mitochondria of HF-fed mice compared to control or MO-fed mice. Moreover, these ROS metrics began to increase at a lower ΔΨ threshold. Similar, but less marked, changes were observed in heart mitochondria of HF-fed mice compared to controls. No changes in mitochondrial bioenergetics were observed in studies of separate mice fed HF versus control for only 12 weeks. In summary, HF feeding of sufficient duration impairs mitochondrial bioenergetics and is associated with a greater ROS “cost” of ATP production compared to controls. These effects are, in part, mitigated by MO.
Details
- Title: Subtitle
- Dietary fat, fatty acid saturation and mitochondrial bioenergetics
- Creators
- Liping Yu - NMR Core Facility and Department of Biochemistry, University of Iowa and the Iowa City Veterans Affairs Medical Center, Iowa City, IA, 52242Brian D Fink - Department of Internal Medicine/Endocrinology, University of Iowa and the Iowa City Veterans Affairs Medical Center, Iowa City, IA, 52242Judith A Herlein - Department of Internal Medicine/Endocrinology, University of Iowa and the Iowa City Veterans Affairs Medical Center, Iowa City, IA, 52242Christine L Oltman - Department of Internal Medicine/Cardiology, University of Iowa and the Iowa City Veterans Affairs Medical Center, Iowa City, IA, 52242Kathryn G Lamping - Department of Pharmacology, University of Iowa and the Iowa City Veterans Affairs Medical Center, Iowa City, IA, 52242William I Sivitz - Department of Internal Medicine/Endocrinology, University of Iowa and the Iowa City Veterans Affairs Medical Center, Iowa City, IA, 52242
- Resource Type
- Journal article
- Publication Details
- Journal of bioenergetics and biomembranes, Vol.46(1), pp.33-44
- DOI
- 10.1007/s10863-013-9530-z
- PMID
- 24121995
- PMCID
- PMC3955408
- NLM abbreviation
- J Bioenerg Biomembr
- ISSN
- 0145-479X
- eISSN
- 1573-6881
- Publisher
- Springer Science and Business Media LLC
- Language
- English
- Date published
- 02/2014
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Biochemistry and Molecular Biology; Medicine Administration; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094319602771
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