Journal article
Dietary fats modify vascular fat composition, eNOS localization within lipid rafts and vascular function in obesity
Physiological reports, Vol.6(15), pp.e13820-n/a
08/2018
DOI: 10.14814/phy2.13820
PMCID: PMC6090220
PMID: 30105819
Abstract
We tested whether dietary fatty acids alter membrane composition shifting localization of signaling pathways within caveolae to determine their role in vascular function. Wild type (
WT
) and caveolin‐1‐deficient mice (cav‐1
KO
), required for vascular caveolae formation, were fed low fat (
LF
), high saturated fat (
HF
, 60% kcal from lard), or high‐fat diet with 50:50 lard and n‐3 polyunsaturated fatty acid‐enriched menhaden oil (
MO
).
HF
and
MO
increased body weight and fat in
WT
but had less effect in cav‐1
KO
.
MO
increased unsaturated fatty acids and the unsaturation index of aorta from
WT
and cav‐1
KO
. In
LF WT
aorta, endothelial nitric oxide synthase (
eNOS
) was localized to cav‐1‐enriched low‐density fractions which shifted to actin‐enriched high‐density fractions with acetylcholine (
AC
h).
HF
and
MO
shifted
eNOS
to high‐density fractions in
WT
aorta which was not affected by
AC
h. In cav‐1
KO
aorta,
eNOS
was localized in low‐density non‐caveolar fractions but not shifted by
AC
h or diet. Inducible
NOS
and cyclooxygenase 1/2 were not localized in low‐density fractions or affected by diet,
AC
h or genotype.
AC
h‐induced dilation of gracilis arteries from
HF WT
was similar to dilation in
LF
but the
NOS
component was reduced. In
WT
and cav‐1
KO
, dilation to
AC
h was enhanced by
MO
through increased role for
NOS
and cyclooxygenase. We conclude that dietary fats affect vascular fatty acid composition and membrane localization of
eNOS
but the contribution of
eNOS
and cyclooxygenase in
AC
h‐mediated vascular responses is independent of lipid rafts.
Details
- Title: Subtitle
- Dietary fats modify vascular fat composition, eNOS localization within lipid rafts and vascular function in obesity
- Creators
- Daniel W Nuno - Roy J. and Lucille A. Carver College of MedicineLawrence J Coppey - Roy J. and Lucille A. Carver College of MedicineMark A Yorek - Roy J. and Lucille A. Carver College of MedicineKathryn G Lamping - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Physiological reports, Vol.6(15), pp.e13820-n/a
- DOI
- 10.14814/phy2.13820
- PMID
- 30105819
- PMCID
- PMC6090220
- NLM abbreviation
- Physiol Rep
- ISSN
- 2051-817X
- eISSN
- 2051-817X
- Publisher
- John Wiley and Sons Inc
- Grant note
- RX000889‐06 / Department of Veterans Affairs to Mark Yorek BX000543‐08 / VA Merit Award to Kathryn Lamping 52246 / Iowa City VA Health Care System
- Alternative title
- D. W. Nuno et al
- Language
- English
- Date published
- 08/2018
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094731202771
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