Differences and similarities in development of corneal nerve damage and peripheral neuropathy and in diet-induced obesity and type 2 diabetic rats

Eric P Davidson; Lawrence J Coppey; Randy H Kardon; Mark A Yorek

doi:10.1167/iovs.13-13794

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Differences and similarities in development of corneal nerve damage and peripheral neuropathy and in diet-induced obesity and type 2 diabetic rats

Journal article

Open access

Peer reviewed

Differences and similarities in development of corneal nerve damage and peripheral neuropathy and in diet-induced obesity and type 2 diabetic rats

Eric P Davidson, Lawrence J Coppey, Randy H Kardon and Mark A Yorek

Investigative ophthalmology & visual science, Vol.55(3), pp.1222-1230

03/03/2014

DOI: 10.1167/iovs.13-13794

PMCID: PMC3941618

PMID: 24519423

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https://doi.org/10.1167/iovs.13-13794View

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Abstract

Peripheral neuropathy has been shown to exist in prediabetic and diabetic patients and animal models. However, the development of peripheral neuropathy in prediabetes and posthyperglycemia is likely different. The purpose of this study was to examine the progression of peripheral neuropathy in diet-induced obese rats and high-fat-fed rats treated with a low dose of streptozotocin, a model for type 2 diabetes, using standard endpoints as well as corneal sensitivity and innervation. Diet-induced obese rats and high-fat/low-dose streptozotocin diabetic rats were used to examine standard peripheral neuropathy endpoints and innervation of the cornea and corneal epithelium using corneal and standard confocal microscopy, respectively, and corneal sensitivity using a Cochet-Bonnet esthesiometer at three different time points. Obese rats and to a greater extent diabetic rats were insulin resistant. Obese and diabetic rats had developed sensory nerve deficits, but only diabetic rats had motor nerve dysfunction as determined by measuring nerve conduction velocity, thermal nociception, and intraepidermal nerve fiber density. In the cornea there was a decrease in corneal nerve fiber length, innervation of the corneal epithelium, and corneal sensitivity in both diet-induced obese and diabetic rats. These studies demonstrate that changes in corneal nerve innervation and sensitivity occur in both obese and type 2 diabetic rat models that are consistent with development of peripheral neuropathy. Examination of corneal nerve changes may be valuable endpoints for exploring potential treatments for peripheral neuropathy in both prediabetes with insulin resistance and diabetes.

Nerve Fibers - pathology

Peripheral Nervous System Diseases - etiology

Sensation

Obesity - complications

Cell Count

Rats

Male

Rats, Sprague-Dawley

Obesity - pathology

Microscopy, Confocal

Animals

Diabetes Mellitus, Experimental - pathology

Cornea - innervation

Cornea - pathology

Cornea - physiopathology

Diabetes Mellitus, Experimental - complications

Peripheral Nervous System Diseases - pathology

Dietary Fats - toxicity

Details

Title: Subtitle: Differences and similarities in development of corneal nerve damage and peripheral neuropathy and in diet-induced obesity and type 2 diabetic rats
Creators: Eric P Davidson - Department of Internal Medicine, The University of Iowa, Iowa City, Iowa
Lawrence J Coppey
Randy H Kardon
Mark A Yorek
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.55(3), pp.1222-1230
DOI: 10.1167/iovs.13-13794
PMID: 24519423
PMCID: PMC3941618
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Grant note: DK073990 / NIDDK NIH HHS R56 DK073990 / NIDDK NIH HHS I01 RX000889 / RRD VA R01 DK073990 / NIDDK NIH HHS
Language: English
Date published: 03/03/2014
Academic Unit: Iowa Neuroscience Institute; Internal Medicine; Ophthalmology and Visual Sciences
Record Identifier: 9983980095002771

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