Differences in the Concentrations of Small, Anionic, Antimicrobial Peptides in Bronchoalveolar Lavage Fluid and in Respiratory Epithelia of Patients with and without Cystic Fibrosis

Kim A Brogden; Mark R Ackermann; Paul B McCray; Kenneth M Huttner

doi:10.1128/IAI.67.8.4256-4259.1999

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Differences in the Concentrations of Small, Anionic, Antimicrobial Peptides in Bronchoalveolar Lavage Fluid and in Respiratory Epithelia of Patients with and without Cystic Fibrosis

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Differences in the Concentrations of Small, Anionic, Antimicrobial Peptides in Bronchoalveolar Lavage Fluid and in Respiratory Epithelia of Patients with and without Cystic Fibrosis

Kim A Brogden, Mark R Ackermann, Paul B McCray and Kenneth M Huttner

Infection and immunity, Vol.67(8), pp.4256-4259

Note

08/1999

DOI: 10.1128/IAI.67.8.4256-4259.1999

PMCID: PMC96733

PMID: 10417200

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Abstract

Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2) antibodies to synthetic H-DDDDDDD-OH, an antimicrobial anionic peptide (AP) originally isolated from ovine pulmonary surfactant, were prepared and used to assess the concentrations of AP-like molecules in human respiratory tract samples. In bronchoalveolar lavage fluids, concentrations of AP-like molecules measured by enzyme-linked immunosorbent assay were significantly lower in 13 patients with cystic fibrosis (CF) (mean ± standard deviation [SD], 0.78 ± 0.46 mM) than in 34 patients without CF (1.30 ± 0.66 mM) ( P = 0.01). In pulmonary tissues of three patients without CF, very little antigen was stained in the apical cytoplasm of the bronchial and bronchiolar epithelium yet robust staining was seen in the alveolar epithelium. In pulmonary tissues of three patients with CF, robust staining of antigen was seen in the apical cytoplasm of the bronchial and bronchiolar epithelium yet no staining was seen in the alveolar epithelium. These results show that AP-like molecules are present in healthy human respiratory tract samples and differ in concentration and location of expression in patients with and without CF.

Host Response and Inflammation

Details

Title: Subtitle: Differences in the Concentrations of Small, Anionic, Antimicrobial Peptides in Bronchoalveolar Lavage Fluid and in Respiratory Epithelia of Patients with and without Cystic Fibrosis
Creators: Kim A Brogden - Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010
Mark R Ackermann - Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010
Paul B McCray - Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010
Kenneth M Huttner - Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.67(8), pp.4256-4259
Publisher: American Society for Microbiology
Series: Note
DOI: 10.1128/IAI.67.8.4256-4259.1999
PMID: 10417200
PMCID: PMC96733
ISSN: 0019-9567
eISSN: 1098-5522
Language: English
Date published: 08/1999
Academic Unit: Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Periodontics; Internal Medicine
Record Identifier: 9984093330102771

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