Different susceptibilities of Leishmania spp. promastigotes to the Annona muricata acetogenins annonacinone and corossolone, and the Platymiscium floribundum coumarin scoparone

Nadja Soares Vila-Nova; Selene Maia de Morais; Maria José Cajazeiras Falcão; Terezinha Thaize Negreiros Alcantara; Pablito Augusto Travassos Ferreira; Eveline Solon Barreira Cavalcanti; Icaro Gusmão Pinto Vieira; Cláudio Cabral Campello; Mary Wilson

doi:10.1016/j.exppara.2012.11.025

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Different susceptibilities of Leishmania spp. promastigotes to the Annona muricata acetogenins annonacinone and corossolone, and the Platymiscium floribundum coumarin scoparone

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Different susceptibilities of Leishmania spp. promastigotes to the Annona muricata acetogenins annonacinone and corossolone, and the Platymiscium floribundum coumarin scoparone

Nadja Soares Vila-Nova, Selene Maia de Morais, Maria José Cajazeiras Falcão, Terezinha Thaize Negreiros Alcantara, Pablito Augusto Travassos Ferreira, Eveline Solon Barreira Cavalcanti, Icaro Gusmão Pinto Vieira, Cláudio Cabral Campello and Mary Wilson

Experimental parasitology, Vol.133(3), pp.334-338

03/2013

DOI: 10.1016/j.exppara.2012.11.025

PMCID: PMC5426951

PMID: 23232251

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Abstract

[Display omitted] ► Acetogenins were isolated from A. muricata and scoparone from P. floribundum. ► All compounds inhibited the promastigote growth in the three species. ► Annonacinone presented high leishmanicidal activity. ► Corossolone, and scoparone demonstrated moderate leishmanicidal actions. ► Toxicity was tested using A. salina assay, corossolone was the most toxic compound. Leishmaniasis is a zoonotic disease that can manifest itself in visceral and cutaneous form. The aim of this study was to search for new leishmanicidal compounds. Preliminarily, Artemia salina assay was applied to compounds from two plants found in Northeastern Brazil, Platymiscium floribundum and Annona muricata. Then these compounds were tested against three Leishmania species (Leishmania donovani, Leishmania mexicana and Leishmania major). A screening assay using luciferase-expressing promastigote form were used to measure the viability of promastigote One coumarin, scoparone, isolated from P. floribundum and two acetogenins, annonacinone and corossolone isolated from A. muricata showed leishmanicidal activity in all species tested. Nevertheless, Leishmania species indicated different susceptibilities in relation to the tested compounds: L. mexicana was more sensitive to scoparone followed by L. major and L. donovani. The three species presented similar inhibition to corossolone and annonacinone. Acetogenin annonacinone (EC50=6.72−8.00μg/mL) indicated high leishmanicidal activity; corossolone (EC50=16.14−18.73μg/mL) and scoparone (EC50=9.11−27.51μg/mL) moderate activity. A. saline larvae were less sensitive to the coumarin scoparone and acetogenin corossolone was the most toxic. In conclusion, the leishmanicidal activity demonstrated by the coumarin and acetogenins indicate these compounds for further studies aiming the development of new leishmanicidal agents.

Leishmanicidal

Artemia salina

Acetogenin

Coumarin

Leishmania

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Title: Subtitle: Different susceptibilities of Leishmania spp. promastigotes to the Annona muricata acetogenins annonacinone and corossolone, and the Platymiscium floribundum coumarin scoparone
Creators: Nadja Soares Vila-Nova - Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Avenida Paranjana, 1700, Campus do Itaperi, 60740-000 Fortaleza, Ceará, Brazil
Selene Maia de Morais - Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Avenida Paranjana, 1700, Campus do Itaperi, 60740-000 Fortaleza, Ceará, Brazil
Maria José Cajazeiras Falcão - Curso de Química, Centro de Ciências e Tecnologia, Universidade Estadual do Ceara, Avenida Paranjana, 1700, Campus do Itaperi, 60740-000 Fortaleza, Ceará, Brazil
Terezinha Thaize Negreiros Alcantara - Curso de Química, Centro de Ciências e Tecnologia, Universidade Estadual do Ceara, Avenida Paranjana, 1700, Campus do Itaperi, 60740-000 Fortaleza, Ceará, Brazil
Pablito Augusto Travassos Ferreira - Curso de Química, Centro de Ciências e Tecnologia, Universidade Estadual do Ceara, Avenida Paranjana, 1700, Campus do Itaperi, 60740-000 Fortaleza, Ceará, Brazil
Eveline Solon Barreira Cavalcanti - Curso de Química, Centro de Ciências e Tecnologia, Universidade Estadual do Ceara, Avenida Paranjana, 1700, Campus do Itaperi, 60740-000 Fortaleza, Ceará, Brazil
Icaro Gusmão Pinto Vieira - PADETEC – Parque de Desenvolvimento Tecnologico, Universidade Federal do Ceará, Av. Humberto Monte, 2977, Bairro Parquelandia Bloco 310, CEP 60.440-593 Fortaleza, Ceará, Brazil
Cláudio Cabral Campello - Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Avenida Paranjana, 1700, Campus do Itaperi, 60740-000 Fortaleza, Ceará, Brazil
Mary Wilson - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Experimental parasitology, Vol.133(3), pp.334-338
DOI: 10.1016/j.exppara.2012.11.025
PMID: 23232251
PMCID: PMC5426951
NLM abbreviation: Exp Parasitol
ISSN: 0014-4894
eISSN: 1090-2449
Publisher: Elsevier Inc
Language: English
Date published: 03/2013
Academic Unit: Microbiology and Immunology; International Programs; Epidemiology; Internal Medicine
Record Identifier: 9984001131402771

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