Different trans RNA splicing events in bloodstream and procyclic Trypanosoma brucei

Jared R Helm; Mary E Wilson; John E Donelson

doi:10.1016/j.molbiopara.2008.02.006

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Different trans RNA splicing events in bloodstream and procyclic Trypanosoma brucei

Journal article

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Different trans RNA splicing events in bloodstream and procyclic Trypanosoma brucei

Jared R Helm, Mary E Wilson and John E Donelson

Molecular and biochemical parasitology, Vol.159(2), pp.134-137

2008

DOI: 10.1016/j.molbiopara.2008.02.006

PMCID: PMC2699461

PMID: 18384893

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https://www.ncbi.nlm.nih.gov/pmc/articles/2699461View

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Abstract

Most trypanosomatid genes are transcribed into polycistronic precursor RNAs that are processed into monocistronic mRNAs possessing a 39-nucleotide spliced leader (SL) at their 5′-ends and polyadenylation at their 3′-ends. We show here that precursor RNA derived from a luciferase gene integrated in reverse orientation at the rDNA locus of Trypanosoma brucei is processed into three major SL-containing RNAs in bloodstream cells and a single SL-containing RNA in procyclic RNAs. This difference in trans RNA splicing between bloodstream and procyclic cells is independent of the 5′- and 3′-UTRs flanking the luciferase coding region. Thus, bloodstream cells can recognize some sequences in precursor RNA as a SL addition site that procyclic cells do not. These alternative SL addition sites may be aberrant or they might be utilized to expand the number of gene products from individual genes. Future experiments on endogenous genes will be necessary to examine the latter possibility.

Trans RNA splicing

Procyclic

Luciferase

Bloodstream

Spliced leader

Trypanosome

Details

Title: Subtitle: Different trans RNA splicing events in bloodstream and procyclic Trypanosoma brucei
Creators: Jared R Helm - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Mary E Wilson - Departments of Internal Medicine, Microbiology and Epidemiology, University of Iowa and VA Medical Center, Iowa City, IA 52242, USA
John E Donelson - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Molecular and biochemical parasitology, Vol.159(2), pp.134-137
DOI: 10.1016/j.molbiopara.2008.02.006
PMID: 18384893
PMCID: PMC2699461
NLM abbreviation: Mol Biochem Parasitol
ISSN: 0166-6851
eISSN: 1872-9428
Publisher: Elsevier B.V
Language: English
Date published: 2008
Academic Unit: Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984001148902771

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