Journal article
Differential DNA methylation of the p16 INK4A/CDKN2A promoter in human oral cancer cells and normal human oral keratinocytes
Oral oncology, Vol.35(5), pp.516-522
1999
DOI: 10.1016/S1368-8375(99)00026-3
PMID: 10694953
Abstract
The
p16 INK4A tumor suppressor gene participates in establishing and maintaining the malignant phenotype of a variety of cancer cell lines and primary tumors. Recently it has been observed that p16 expression is lost in oral cavity cancer cell lines in the presence of a normal intact gene. To examine the role of DNA methylation as an explanation for these findings, we analyzed the DNA methylation patterns of the
p16 INK4A promoter in DNA isolated from primary cultures of normal human oral keratinocytes and squamous cell carcinoma (SCC-15) oral cancer cells using bisulfite genomic sequencing. Our results demonstrated striking differences in the methylation status of the 5′ CpG island of the
p16 gene between normal and cancer cells. Normal human oral keratinocytes showed practically no methylation of the
p16 INK4A promoter, while SCC-15 oral cancer cells showed almost complete methylation in this region. These data implicate DNA methylation as a mechanism for transcriptional silencing of the
p16 INK4A gene in oral cancer cells.
Details
- Title: Subtitle
- Differential DNA methylation of the p16 INK4A/CDKN2A promoter in human oral cancer cells and normal human oral keratinocytes
- Creators
- D.T Cody - Radiation Biology Graduate Program, College of Medicine, The University of Iowa, Iowa City, IA 52242, USAYuanhui Huang - Radiation Biology Graduate Program, College of Medicine, The University of Iowa, Iowa City, IA 52242, USAC.J Darby - Radiation Biology Graduate Program, College of Medicine, The University of Iowa, Iowa City, IA 52242, USAG.K Johnson - Dows Institute, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USAF.E Domann - Radiation Biology Graduate Program, College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Oral oncology, Vol.35(5), pp.516-522
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/S1368-8375(99)00026-3
- PMID
- 10694953
- ISSN
- 1368-8375
- eISSN
- 1879-0593
- Language
- English
- Date published
- 1999
- Academic Unit
- Pathology; Surgery; Radiation Oncology; Periodontics
- Record Identifier
- 9984047632202771
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