Journal article
Differential Effects of Myocilin and Optineurin, Two Glaucoma Genes, on Neurite Outgrowth
The American journal of pathology, Vol.176(1), pp.343-352
01/01/2010
DOI: 10.2353/ajpath.2010.090194
PMCID: PMC2797895
PMID: 19959812
Abstract
Myocilin
and
optineurin
are two genes linked to glaucoma, a major blinding disease characterized by progressive loss of retinal ganglion cells (RGCs) and their axons. To investigate the effects of force-expressed wild-type and mutant myocilin and optineurin on neurite outgrowth in neuronal cells, we transiently transfected cells with pEGFP-N1 (mock control) as well as
myocilin
and
optineurin
plasmids including pMYOC
WT
-EGFP, pMYOC
P370L
-EGFP, pMYOC
1-367
-EGFP, pOPTN
WT
-EGFP, and pOPTN
E50K
-EGFP. PC12 cells transfected with pEGFP-N1 produced, as anticipated, long and extensive neuritis on nerve growth factor induction. The neurite length in those cells transfected with
myocilin
constructs was shortened and the number of neurites was also reduced. A similar inhibitory effect on neurite outgrowth was also elicited by
myocilin
transfection in RGC5 cells. In contrast, neither transfection of the
optineurin
constructs pOPTN
WT
-EGFP and pOPTN
E50K
-EGFP nor the myocilin and optineurin small-interfering RNA treatments induced significant alterations in neurite outgrowth. Transfection with the wild-type
optineurin
construct, but not with that of the wild-type
myocilin
, increased the apoptotic activity in cells. These results demonstrated that the two glaucoma genes,
myocilin
and
optineurin
, exhibited differential effects on neurite outgrowth. They may contribute to the development of neurodegenerative glaucoma via distinct mechanisms.
Details
- Title: Subtitle
- Differential Effects of Myocilin and Optineurin, Two Glaucoma Genes, on Neurite Outgrowth
- Creators
- Takahisa Koga - University of Illinois ChicagoXiang Shen - University of Illinois Urbana-ChampaignJeong-Seok Park - University of Illinois ChicagoYe Qiu - University of Illinois Urbana-ChampaignBum-Chan Park - University of Illinois ChicagoRajalekshmy Shyam - University of Illinois Urbana-ChampaignBeatrice Y.J.T. Yue - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- The American journal of pathology, Vol.176(1), pp.343-352
- DOI
- 10.2353/ajpath.2010.090194
- PMID
- 19959812
- PMCID
- PMC2797895
- NLM abbreviation
- Am J Pathol
- ISSN
- 0002-9440
- eISSN
- 1525-2191
- Publisher
- American Society for Investigative Pathology
- Number of pages
- 10
- Language
- English
- Date published
- 01/01/2010
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984949237802771
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