Differential Regulation of Hepatic Heme Oxygenase-1 Protein With Aging and Heat Stress

Steven A Bloomer; Hannah J Zhang; Kyle E Brown; Kevin C Kregel

doi:10.1093/gerona/gln056

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Differential Regulation of Hepatic Heme Oxygenase-1 Protein With Aging and Heat Stress

Journal article

Open access

Peer reviewed

Differential Regulation of Hepatic Heme Oxygenase-1 Protein With Aging and Heat Stress

Steven A Bloomer, Hannah J Zhang, Kyle E Brown and Kevin C Kregel

The journals of gerontology. Series A, Biological sciences and medical sciences, Vol.64A(4), pp.419-425

04/2009

DOI: 10.1093/gerona/gln056

PMCID: PMC2657177

PMID: 19196643

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Abstract

Increased expression of heme oxygenase-1 (HO-1) in response to physiological stress is considered to be a protective response, which may be altered with aging. In this study, HO-1 expression was assessed following heat stress by immunoblotting of liver homogenates and isolated hepatocytes from young (6 months) and old (24 months) Fischer 344 rats and by immunohistochemistry. Livers of old rats showed higher baseline levels of HO-1, which was predominately localized to Kupffer cells. After heat stress, young animals showed a greater relative increase in hepatic HO-1, part of which was caused by increased numbers of nonparenchymal cells that were immunoreactive to HO-1. Consistent with these data, HO-1 was significantly upregulated after hyperthermia in vitro only in hepatocytes from young rats. Hence, aging alters stress-induced expression of HO-1 in a cell-specific manner, which may contribute to the diminished stress tolerance observed in older organisms.

Biological Sciences

Oxidative stress

Journal of Gerontology

Inflammation

Hyperthermia

Kupffer cells

Details

Title: Subtitle: Differential Regulation of Hepatic Heme Oxygenase-1 Protein With Aging and Heat Stress
Creators: Steven A Bloomer - Department of Integrative Physiology, University of Iowa, Iowa City
Hannah J Zhang - Department of Integrative Physiology, University of Iowa, Iowa City
Kyle E Brown - Iowa City Veterans Administration Medical Center, Iowa City
Kevin C Kregel - Department of Integrative Physiology, University of Iowa, Iowa City
Resource Type: Journal article
Publication Details: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol.64A(4), pp.419-425
DOI: 10.1093/gerona/gln056
PMID: 19196643
PMCID: PMC2657177
NLM abbreviation: J Gerontol A Biol Sci Med Sci
ISSN: 1079-5006
eISSN: 1758-535X
Publisher: Oxford University Press
Language: English
Date published: 04/2009
Academic Unit: Gastroenterology and Hepatology; Radiation Oncology; Provost Office Administration; Health, Sport, and Human Physiology ; Internal Medicine
Record Identifier: 9984046822302771

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