Differential binding to and biofilm formation on, HEp-2 cells by Salmonella enterica serovar Typhimurium is dependent upon allelic variation in the fimH gene of the fim gene cluster

Jennifer D Boddicker; Nathan A Ledeboer; Jennifer Jagnow; Bradley D Jones; Steven Clegg

doi:10.1046/j.1365-2958.2002.03121.x

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Differential binding to and biofilm formation on, HEp-2 cells by Salmonella enterica serovar Typhimurium is dependent upon allelic variation in the fimH gene of the fim gene cluster

Journal article

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Peer reviewed

Differential binding to and biofilm formation on, HEp-2 cells by Salmonella enterica serovar Typhimurium is dependent upon allelic variation in the fimH gene of the fim gene cluster

Jennifer D Boddicker, Nathan A Ledeboer, Jennifer Jagnow, Bradley D Jones and Steven Clegg

Molecular microbiology, Vol.45(5), pp.1255-1265

09/2002

DOI: 10.1046/j.1365-2958.2002.03121.x

PMID: 12207694

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Abstract

Type 1 fimbria-mediated adherence to HEp-2 cells by two strains of Salmonella enterica serovar Typhimurium was found to be different. Although both strains exhibited a strong mannose-sensitive haemagglutination reaction with guinea pig erythrocytes, characteristic of the expression of type 1 fimbriae, only one of the strains adhered in large numbers to HEp-2 cells. Characterization of the fimH genes, encoding the fimbrial adhesins, indicated two allelic variants. Using fimH mutants of the two strains it was possible to demonstrate that binding to HEp-2 cells was associated with the presence of one of the alleles regardless of the host strain. Therefore, this differential binding was not a function of the type I fimbrial shaft or the presence of other types of fimbriae produced by one strain but not the other. These observations may explain the differences in HEp-2 binding by type 1 fimbriate strains of Salmonella previously reported by several groups. Also, our studies demonstrate that the FimH adhesin can influence the efficiency of biofilm formation on HEp-2 cells using once-flow-through continuous culture conditions. The formation of biofilms on eukaryotic cells using this procedure is more likely to represent those conditions found in the intestinal tract than conditions using batch culture techniques to investigate adherence and biofilm formation. Indeed, the increased efficiency of biofilm formation in the murine intestinal tract confirmed the role of one of the fimH alleles in this process.

Fimbriae Proteins

Multigene Family

Adhesins, Bacterial - genetics

Humans

Biofilms - growth & development

Fimbriae, Bacterial - genetics

Genetic Variation

Bacterial Adhesion - physiology

Base Sequence

Female

Transformation, Genetic

Cell Line

Genes, Bacterial

Salmonella typhimurium - physiology

Guinea Pigs

Mutagenesis, Site-Directed

Salmonella typhimurium - genetics

Adhesins, Escherichia coli

Hemagglutination

Animals

DNA, Bacterial - genetics

Intestines - microbiology

Fimbriae, Bacterial - physiology

Alleles

Bacterial Adhesion - genetics

Mice

Mice, Inbred BALB C

In Vitro Techniques

Details

Title: Subtitle: Differential binding to and biofilm formation on, HEp-2 cells by Salmonella enterica serovar Typhimurium is dependent upon allelic variation in the fimH gene of the fim gene cluster
Creators: Jennifer D Boddicker - Department of Microbiology, College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Nathan A Ledeboer
Jennifer Jagnow
Bradley D Jones
Steven Clegg
Resource Type: Journal article
Publication Details: Molecular microbiology, Vol.45(5), pp.1255-1265
DOI: 10.1046/j.1365-2958.2002.03121.x
PMID: 12207694
NLM abbreviation: Mol Microbiol
ISSN: 0950-382X
eISSN: 1365-2958
Language: English
Date published: 09/2002
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083813702771

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